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Betaine-Homocysteine S-Methyltransferase Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644525

Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from… More details

$785 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Betaine-homocysteine S-methyltransferase, Polyclonal Antibody
Full Product Name
Betaine-homocysteine S-methyltransferase, CT (BHMT)
Product Synonym Names
Anti -Betaine-homocysteine S-methyltransferase, CT (BHMT)
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human
Form/Format
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human BHMT. Species sequence homology: Canine, mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-EQQLKELFEKQK from human BHMT at C-terminal.
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Dilution: ELISA: 1:16,000
Western Blot: 1:16,000, 0.1-0.3ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml

Immunohistochemistry (IHC) of anti-Betaine-homocysteine S-methyltransferase antibody

Human Kidney (formalin-fixed, paraffin-embedded) stained with BHMT antibody at 2.5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.
Goat Anti-Betaine-Homocysteine S-Methyltransferase Polyclonal Antibody – Cat# MBS644525

Immunohistochemistry (IHC) of anti-Betaine-homocysteine S-methyltransferase antibody

Human Liver (formalin-fixed, paraffin-embedded) stained with BHMT antibody at 2.5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.
Goat Anti-Betaine-Homocysteine S-Methyltransferase Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644525

Testing Data of anti-Betaine-homocysteine S-methyltransferase antibody

Antibody (0.03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-Betaine-Homocysteine S-Methyltransferase Polyclonal Antibody, Testing Data – Cat# MBS644525

Other Notes

Small volumes of anti-Betaine-homocysteine S-methyltransferase antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Betaine-homocysteine S-methyltransferase purchase

MBS644525 is an antibody targeting Betaine-homocysteine S-methyltransferase. Also known as Betaine-homocysteine S-methyltransferase, CT (BHMT). Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for Betaine-homocysteine S-methyltransferase. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
betaine-homocysteine S-methyltransferase

UniProt Comments for Betaine-homocysteine S-methyltransferase

BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Protein type: EC 2.1.1.5; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase

Cellular Component: protein complex; cytoplasm; cytosol

Molecular Function: betaine-homocysteine S-methyltransferase activity; methyltransferase activity; zinc ion binding; protein complex binding

Biological Process: methylation; S-methylmethionine cycle; protein amino acid methylation; methionine biosynthetic process; betaine metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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