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USP14 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644528

MBS644528 is an antibody targeting USP14. Also known as USP14 (Ubiquitin-specific-processing Protease 14, Ubiquitin Carboxyl-terminal Hydrolase 14, Deubiquitinating Enzyme 14, Ubiquitin Thioesterase 14, TGT). Reactive in Human, Mouse, Rat. Host: Mouse. More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
USP14, Monoclonal Antibody
Full Product Name
USP14 (Ubiquitin-specific-processing Protease 14, Ubiquitin Carboxyl-terminal Hydrolase 14, Deubiquitinating Enzyme 14, Ubiquitin Thioesterase 14, TGT)
Product Gene Name
anti-USP14 antibody
Product Synonym Names
Anti -USP14 (Ubiquitin-specific-processing Protease 14, Ubiquitin Carboxyl-terminal Hydrolase 14, Deubiquitinating Enzyme 14, Ubiquitin Thioesterase 14, TGT)
Immunogen Sequence
PQKEVKYEPF SFADDIGSNN CGYYDLQAVL THQGRSSSSG HYVSWVKRKQ DEWIKFDDDK VSIVTPEDIL RLSGGGDWHI AYVLLYGPRR VEIMEEESEQ
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
6D6
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human USP14. Species Crossreactivity: mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
56,069 Da
Chromosome Location
Chromosome: 18; NC_000018.9 (158483..213739). Location: 18p11.32
OMIM
607274
3D Structure
ModBase 3D Structure for P54578
Immunogen
Partial recombinant corresponding to aa395-495 from USP14 (NP_005142) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-USP14 antibody

Antibodies; Abs to Ubiquitin
Applications Tested/Suitable for anti-USP14 antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-USP14 antibody
Suitable for use in ELISA, Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 20ug/ml

Immunofluorescence (IF) of anti-USP14 antibody

Immunofluorescence of monoclonal antibody to USP14 on HeLa cell. [antibody concentration 20ug/ml]
Mouse Anti-USP14 Monoclonal Antibody – Cat# MBS644528

Testing Data of anti-USP14 antibody

Detection limit for recombinant GST tagged USP14 is ~0.1ng/ml as a capture antibody.
Mouse Anti-USP14 Monoclonal Antibody, Testing Data – Cat# MBS644528

Western Blot (WB) of anti-USP14 antibody

Western Blot analysis of USP14 expression in transfected 293T cell line by USP14 monoclonal antibody.|Lane 1: USP14 transfected lysate (56.1kD).|Lane 2: Non-transfected lysate.
Mouse Anti-USP14 Monoclonal Antibody, Western Blot (WB) – Cat# MBS644528

Western Blot (WB) of anti-USP14 antibody

USP14 monoclonal antibody Western Blot analysis of USP14 expression in NIH/3T3 .
Mouse Anti-USP14 Monoclonal Antibody, Western Blot (WB) – Cat# MBS644528

Western Blot (WB) of anti-USP14 antibody

USP14 monoclonal antibody Western Blot analysis of USP14 expression in PC-12.
Mouse Anti-USP14 Monoclonal Antibody, Western Blot (WB) – Cat# MBS644528

Other Notes

Small volumes of anti-USP14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for USP14. It may not necessarily be applicable to this product.

NCBI GI #
82880645
NCBI GeneID
9097
NCBI Accession #
NP_001032411.1
UniProt Primary Accession #
P54578
UniProt Secondary Accession #
Q53XY5; J3QRZ5
UniProt Related Accession #
P54578
GenBank Nucleotide #
NM_001037334.1
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 14 isoform b
NCBI Official Synonym Full Names
ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
NCBI Official Symbol
USP14
NCBI Official Synonym Symbols
TGT
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 14
UniProt Synonym Protein Names
Deubiquitinating enzyme 14; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14
UniProt Gene Name
USP14
UniProt Synonym Gene Names
TGT
UniProt Entry Name
UBP14_HUMAN
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 14; ubiquitin thioesterase 14; deubiquitinating enzyme 14; ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; ubiquitin-specific-processing protease 14; tRNA-guanine transglycosylase, 60-kD subunit; ubiquitin specific protease 14 (tRNA-guanine transglycosylase)

NCBI Summary for USP14

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for USP14

USP14: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Homodimer (Potential). Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1. Belongs to the peptidase C19 family. USP14/UBP6 subfamily.

Protein type: EC 3.4.19.12; Ubiquitin conjugating system; Ubiquitin-specific protease; Protease

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: proteasome complex; cell surface; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; synapse; nucleus

Molecular Function: tRNA guanylyltransferase activity; protein binding; cysteine-type endopeptidase activity; endopeptidase inhibitor activity; ubiquitin-specific protease activity

Biological Process: synaptic transmission; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of chemotaxis
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Research Articles on USP14

1. overexpression of HA-USP14 increased the LPS-, TNFalpha-, or Escherichia coli-induced IL-8 release in human lung epithelial cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-USP14 antibody

 Reference Product  PubMed Publications
 UCHL5 antibody  >8 publications with USP14 and UCHL5

Diseases associated with anti-USP14 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >7 publications with USP14 and Neoplasms
 Disease Models, Animal Antibodies  >4 publications with USP14 and Disease Models, Animal
 Ovarian Diseases Antibodies  >2 publications with USP14 and Ovarian Diseases
 Brain Diseases Antibodies  >2 publications with USP14 and Brain Diseases
 Inflammation Antibodies  >2 publications with USP14 and Inflammation
 Liver Neoplasms Antibodies  >2 publications with USP14 and Liver Neoplasms
 Atrophy Antibodies  >2 publications with USP14 and Atrophy
 Liver Diseases Antibodies  >2 publications with USP14 and Liver Diseases
 Immune System Diseases Antibodies  >1 publications with USP14 and Immune System Diseases
 Weight Loss Antibodies  >1 publications with USP14 and Weight Loss

Organs/Tissues associated with anti-USP14 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7 publications with USP14 and Brain
 Lung Antibodies  >2 publications with USP14 and Lung
 Testis Antibodies  >1 publications with USP14 and Testis
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