• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GLD2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644631

GLD2 is cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. GLD2 does not… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GLD2, Polyclonal Antibody
Full Product Name
GLD2, NT (PAPD4, GLD2, Poly(A) RNA polymerase GLD2, PAP-associated domain-containing protein 4, Terminal uridylyltransferase 2)
Product Gene Name
anti-Gld2 antibody
Product Synonym Names
Anti -GLD2, NT (PAPD4, GLD2, Poly(A) RNA polymerase GLD2, PAP-associated domain-containing protein 4, Terminal uridylyltransferase 2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Hamster, Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, hamster, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
147,592 Da
Chromosome Location
Chromosome: 3R; NT_033777.2 (18037546..18043137). Location: 94A3-94A3
3D Structure
ModBase 3D Structure for Q9VD44
Immunogen
GLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-95 amino acids from the N-terminal region of human GLD2.

Product Categories/Family for anti-Gld2 antibody

Antibodies; Abs to Enzymes; Polymerase
Applications Tested/Suitable for anti-Gld2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Gld2 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Western Blot (WB) of anti-Gld2 antibody

(LEFT)Western blot analysis of GLD2 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(MIDDLE)Western blot analysis of GLD2 Antibody (N-term) in mouse heart tissue lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of GLD2 Antibody (N-term) in CHO cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.
Rabbit Anti-GLD2 Polyclonal Antibody – Cat# MBS644631

Immunohistochemistry (IHC) of anti-Gld2 antibody

GLD2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-GLD2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644631

Other Notes

Small volumes of anti-Gld2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Gld2 purchase

MBS644631 is an antibody targeting Gld2. Also known as GLD2, NT (PAPD4, GLD2, Poly(A) RNA polymerase GLD2, PAP-associated domain-containing protein 4, Terminal uridylyltransferase 2). Reactive in Hamster, Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Gld2. It may not necessarily be applicable to this product.

NCBI GI #
442620418
NCBI GeneID
42602
NCBI Accession #
NP_001262829.1
UniProt Primary Accession #
Q9VD44
UniProt Secondary Accession #
Q8MR77; B5RJK9
UniProt Related Accession #
Q9VD44
GenBank Nucleotide #
NM_001275900.1
NCBI Official Full Name
Gld2, isoform C
NCBI Official Symbol
Gld2
NCBI Official Synonym Symbols
CG5732; Dm1; DmCG5732; DmelCG5732; DmGLD2
UniProt Protein Name
Poly(A) RNA polymerase gld-2 homolog A
Protein Family
Glycerol 2-dehydrogenase
UniProt Gene Name
Gld2
UniProt Synonym Gene Names
DmGLD2
UniProt Entry Name
GLD2A_DROME
NCBI Protein Information
CG5732 gene product from transcript CG5732-RC; CG5732-PB; CG5732-PC; Gld2; Gld2-PB; Gld2-PC

UniProt Comments for Gld2

Function: Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Required for formation of long term memory. Ref.5

Catalytic activity: ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor: Magnesium or manganese

By similarity.

Subunit structure: Interacts with Fmr1 and eIF-4E. Ref.5

Subcellular location: Cytoplasm. Nucleus. Note: Found in neuronal cytoplasm, present in discrete neuronal particles associated with mRNA control, and in nuclear puncta. Ref.5

Tissue specificity: Expressed in the brain. Ref.5

Developmental stage: Expressed in larvae, pupae and adults. Ref.5

Sequence similarities: Belongs to the DNA polymerase type-B-like family. GLD2 subfamily.Contains 1 PAP-associated domain.
Show more

Research Articles on Gld2

1. Results indicate that GLD2 is an important regulator of late spermatogenesis and is the first example of a Gld-2 family member that plays a significant role specifically in male gametogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-Gld2 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with Gld2 and Testis
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000