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BAG2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644807

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BAG2, Polyclonal Antibody
Full Product Name
BAG2, CT (BAG2, BAG family molecular chaperone regulator 2, Bcl-2-associated athanogene 2)
Product Gene Name
Product Synonym Names
Anti -BAG2, CT (BAG2, BAG family molecular chaperone regulator 2, Bcl-2-associated athanogene 2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
23,772 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (57037104..57050013). Location: 6p12.1-p11.2
OMIM
603882
3D Structure
ModBase 3D Structure for O95816
Immunogen
BAG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-176 amino acids from the C-terminal region of human BAG2.

Product Categories/Family for anti-BAG2 antibody

Applications Tested/Suitable for anti-BAG2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-BAG2 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Western Blot (WB) of anti-BAG2 antibody

Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) using MBS644807.This demonstrates the BAG2 antibody detected the BAG2 protein (arrow).
Rabbit Anti-BAG2 Polyclonal Antibody – Cat# MBS644807

Immunohistochemistry (IHC) of anti-BAG2 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human ovarian carcinoma using MBS644807 followed by peroxidase conjugation of the secondary antibody and DAB staining.
Rabbit Anti-BAG2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644807

Other Notes

Small volumes of anti-BAG2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BAG2 purchase

MBS644807 is an antibody targeting BAG2. Also known as BAG2, CT (BAG2, BAG family molecular chaperone regulator 2, Bcl-2-associated athanogene 2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BAG2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
BAG family molecular chaperone regulator 2
NCBI Official Synonym Full Names
BCL2-associated athanogene 2
NCBI Official Symbol
BAG2
NCBI Official Synonym Symbols
BAG-2; dJ417I1.2
UniProt Protein Name
BAG family molecular chaperone regulator 2
UniProt Synonym Protein Names
Bcl-2-associated athanogene 2
UniProt Gene Name
BAG2
UniProt Synonym Gene Names
BAG-2
UniProt Entry Name
BAG2_HUMAN
NCBI Protein Information
BAG family molecular chaperone regulator 2; bcl-2-associated athanogene 2; BAG-family molecular chaperone regulator-2; dJ417I1.2 (BAG-family molecular chaperone regulator 2)

NCBI Summary for BAG2

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]
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UniProt Comments for BAG2

BAG2: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 6p12.1-p11.2

Molecular Function: identical protein binding; protein binding; chaperone binding

Biological Process: protein folding; protein metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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