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RAB3D Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9213520

RAB3D is a protein transport. Probably involved in regulated exocytosis (By similarity). More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RAB3D, Polyclonal Antibody
Full Product Name
RAB3D Antibody (C-term)
Product Gene Name
Product Synonym Names
Ras-related protein Rab-3D; RAB3D; GOV; RAB16
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Concentration
Vial Concentration: 0.5 (lot specific)
Specificity
This RAB3D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-212 amino acids from the C-terminal region of human RAB3D.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
RAB3D peptide (MBS9219755) is used for blocking the activity of RAB3D antibody (MBS9213520)
Molecular Weight
24267
Immunogen Sequence Positions
185-212
OMIM
604350
3D Structure
ModBase 3D Structure for O95716
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Applications Tested/Suitable for anti-RAB3D antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-RAB3D antibody
WB~~1:1000

Western Blot (WB) of anti-RAB3D antibody

RAB3D Antibody (C-term) western blot analysis in K562,HL-60,MDA-MB231,MDA-MB453,CEM cell line lysates (35ug/lane).This demonstrates the RAB3D antibody detected the RAB3D protein (arrow).
Rabbit Anti-RAB3D Polyclonal Antibody – Cat# MBS9213520

Immunohistochemistry (IHC) of anti-RAB3D antibody

RAB3D Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RAB3D Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-RAB3D Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9213520

Flow Cytometry (FC/FACS) of anti-RAB3D antibody

RAB3D Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-RAB3D Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9213520

Product References and Citations for anti-RAB3D antibody

Tian, X., et al. Mol. Cell. Biol. 30(5):1269-1284(2010)
Knop, M., et al. EMBO J. 23(15):2982-2992(2004)
Matsumoto, M., et al. Proc. Natl. Acad. Sci. U.S.A. 101(22):8313-8318(2004)
Nguyen, D., et al. J. Cell. Physiol. 197(3):400-408(2003)
Fukuda, M. J. Biol. Chem. 278(17):15373-15380(2003)

Other Notes

Small volumes of anti-RAB3D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RAB3D purchase

MBS9213520 is an antibody targeting RAB3D. Also known as RAB3D Antibody (C-term). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RAB3D. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ras-related protein Rab-3D
NCBI Official Synonym Full Names
RAB3D, member RAS oncogene family
NCBI Official Symbol
RAB3D
NCBI Official Synonym Symbols
GOV; D2-2; RAB16; RAD3D
UniProt Protein Name
Ras-related protein Rab-3D
Protein Family
UniProt Gene Name
RAB3D
UniProt Synonym Gene Names
GOV; RAB16
UniProt Entry Name
RAB3D_HUMAN
NCBI Protein Information
ras-related protein Rab-3D

UniProt Comments for RAB3D

RAB3D: Protein transport. Probably involved in regulated exocytosis. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric, Rab; G protein, monomeric

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: synaptic vesicle; transport vesicle; mitochondrion; secretory granule membrane; plasma membrane; endosome

Molecular Function: GTPase activity; GTPase binding; GDP binding; GTP binding; myosin V binding

Biological Process: regulation of exocytosis; intracellular protein transport; synaptic vesicle exocytosis; protein secretion; peptidyl-cysteine methylation; Rab protein signal transduction; vesicle docking during exocytosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RAB3D antibody

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