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ACTN3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644967

Alpha-actinin is an actin-binding protein with multiple roles in different cell types. ACTN3 expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments. More details

$935 / 0.05 mg +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ACTN3, Polyclonal Antibody
Full Product Name
ACTN3, NT (Alpha-actinin-3, Alpha-actinin Skeletal Muscle Isoform 3, F-actin Cross-linking Protein)
Product Gene Name
Product Synonym Names
Anti -ACTN3, NT (Alpha-actinin-3, Alpha-actinin Skeletal Muscle Isoform 3, F-actin Cross-linking Protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ACTN3. Species sequence homology: mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
103,241 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (66314312..66330799). Location: 11q13.1
OMIM
102574
3D Structure
ModBase 3D Structure for Q08043
Immunogen
Synthetic peptide corresponding to human ACTN3.
Applications Tested/Suitable for anti-ACTN3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ACTN3 antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:20,000
Western Blot: 1:500-1:1000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml

Immunohistochemistry (IHC) of anti-ACTN3 antibody

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Rabbit Anti-ACTN3 Polyclonal Antibody – Cat# MBS644967

Western Blot (WB) of anti-ACTN3 antibody

Western blot analysis of extracts from HT-29 cells, using ACTN3 Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-ACTN3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS644967

Other Notes

Small volumes of anti-ACTN3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTN3 purchase

MBS644967 is an antibody targeting ACTN3. Also known as ACTN3, NT (Alpha-actinin-3, Alpha-actinin Skeletal Muscle Isoform 3, F-actin Cross-linking Protein). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTN3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-actinin-3 isoform 2
NCBI Official Synonym Full Names
actinin, alpha 3
NCBI Official Symbol
ACTN3
UniProt Protein Name
Alpha-actinin-3
UniProt Synonym Protein Names
Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein
Protein Family
UniProt Gene Name
ACTN3
UniProt Entry Name
ACTN3_HUMAN
NCBI Protein Information
alpha-actinin-3; F-actin cross-linking protein; alpha-actinin skeletal muscle

NCBI Summary for ACTN3

This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants. The reference genome contains the non-coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Apr 2012]
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UniProt Comments for ACTN3

ACTN3: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Belongs to the alpha-actinin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: focal adhesion; actin filament; pseudopodium; cytosol

Molecular Function: integrin binding; protein binding; protein homodimerization activity; structural constituent of muscle; calcium ion binding; actin binding

Biological Process: focal adhesion formation; regulation of apoptosis; muscle filament sliding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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