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RDH10 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645587

RDH10 generates all-trans retinal from all-trans retinol and may plan an important role in the photic visual cycle. All-trans retinal is isomerized to 11-cis retinal by the retinal G protein-coupled receptor (RGR; MIM 600342) when the retinal pigment… More details

$895 / 0.2 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RDH10, Polyclonal Antibody
Full Product Name
RDH10, ID (RDH10, Retinol dehydrogenase 10)
Product Gene Name
Product Synonym Names
Anti -RDH10, ID (RDH10, Retinol dehydrogenase 10)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
38,087 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (74206837..74237520). Location: 8q21.11
OMIM
607599
3D Structure
ModBase 3D Structure for Q8IZV5
Immunogen
RDH10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113~143 amino acids from the Central region of human RDH10.
Applications Tested/Suitable for anti-RDH10 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-RDH10 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-RDH10 antibody

RDH10 Antibody (Center) western blot analysis in WiDr(lane 1),NCI-H460(lane 2),HepG2(lane 3) cell line lysates (35ug/lane).This demonstrates the RDH10 antibody detected the RDH10 protein (arrow).
Rabbit Anti-RDH10 Polyclonal Antibody – Cat# MBS645587

Immunohistochemistry (IHC) of anti-RDH10 antibody

RDH10 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RDH10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-RDH10 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS645587

Flow Cytometry (FC/FACS) of anti-RDH10 antibody

RDH10 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-RDH10 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS645587

Other Notes

Small volumes of anti-RDH10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RDH10 purchase

MBS645587 is an antibody targeting RDH10. Also known as RDH10, ID (RDH10, Retinol dehydrogenase 10). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RDH10. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
retinol dehydrogenase 10
NCBI Official Synonym Full Names
retinol dehydrogenase 10 (all-trans)
NCBI Official Symbol
RDH10
NCBI Official Synonym Symbols
SDR16C4
UniProt Protein Name
Retinol dehydrogenase 10
Protein Family
UniProt Gene Name
RDH10
UniProt Entry Name
RDH10_HUMAN
NCBI Protein Information
retinol dehydrogenase 10; short chain dehydrogenase/reductase family 16C, member 4

NCBI Summary for RDH10

This gene encodes a retinol dehydrogenase, which converts all-trans-retinol to all-trans-retinal, with preference for NADP as a cofactor. Studies in mice suggest that this protein is essential for synthesis of embryonic retinoic acid and is required for limb, craniofacial, and organ development. [provided by RefSeq, Dec 2011]
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UniProt Comments for RDH10

Function: Retinol dehydrogenase with a clear preference for NADP. Converts all-trans-retinol to all-trans-retinal. Has no detectable activity towards 11-cis-retinol, 9-cis-retinol and 13-cis-retinol. Ref.1

Catalytic activity: All-trans-retinol + NADP+ = all-trans-retinal + NADPH. Ref.1

Pathway: Cofactor metabolism; retinol metabolism.

Subcellular location: Microsome membrane; Single-pass membrane protein

Potential. Endoplasmic reticulum membrane; Single-pass membrane protein

Potential Ref.1.

Tissue specificity: Detected in retina, kidney, liver, small intestine, placenta, lung, heart and skeletal muscle. Ref.1 Ref.4

Sequence similarities: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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