• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SMPD2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645788

SMPD2 converts sphingomyelin to ceramide and hydrolyzes 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SMPD2, Polyclonal Antibody
Full Product Name
SMPD2, NT (SMPD2, Sphingomyelin phosphodiesterase 2, Lyso-platelet-activating factor-phospholipase C, Neutral sphingomyelinase)
Product Gene Name
Product Synonym Names
Anti -SMPD2, NT (SMPD2, Sphingomyelin phosphodiesterase 2, Lyso-platelet-activating factor-phospholipase C, Neutral sphingomyelinase)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
47,645 Da
Chromosome Location
Chromosome: 20; NC_005119.3 (47904390..47907503, complement). Location: 20q12
3D Structure
ModBase 3D Structure for Q9ET64
Immunogen
SMPD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~44 amino acids from the N-terminal region of human SMPD2.
Applications Tested/Suitable for anti-Smpd2 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-Smpd2 antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-Smpd2 antibody

Western blot analysis of SMPD2 Antibody (N-term) in mouse lung tissue lysates (35ug/lane). SMPD2 (arrow) was detected using the purified Pab.
Rabbit Anti-SMPD2 Polyclonal Antibody – Cat# MBS645788

Flow Cytometry (FC/FACS) of anti-Smpd2 antibody

SMPD2 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-SMPD2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS645788

Other Notes

Small volumes of anti-Smpd2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Smpd2 purchase

MBS645788 is an antibody targeting Smpd2. Also known as SMPD2, NT (SMPD2, Sphingomyelin phosphodiesterase 2, Lyso-platelet-activating factor-phospholipase C, Neutral sphingomyelinase). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Smpd2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sphingomyelin phosphodiesterase 2
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase 2, neutral membrane
NCBI Official Symbol
Smpd2
NCBI Official Synonym Symbols
nSMase
UniProt Protein Name
Sphingomyelin phosphodiesterase 2
UniProt Synonym Protein Names
Lyso-platelet-activating factor-phospholipase C; Lyso-PAF-PLC; Neutral sphingomyelinase
UniProt Gene Name
Smpd2
UniProt Synonym Gene Names
Lyso-PAF-PLC; N-SMase; nSMase
UniProt Entry Name
NSMA_RAT
NCBI Protein Information
sphingomyelin phosphodiesterase 2; N-SMase; lyso-PAF-PLC; neutral sphingomyelinase; lyso-platelet-activating factor-phospholipase C

NCBI Summary for Smpd2

catalyzes the Mg2+ dependent hydrolysis of sphingomyelin to form N-acylsphingosine and choline phosphate; also has a lower activity for the hydrolysis of lyso-platelet activating factor to 1-alkylglycerol [RGD, Feb 2006]

UniProt Comments for Smpd2

Function: Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.

Catalytic activity: Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.

Cofactor: Magnesium.

Pathway: Lipid metabolism; sphingolipid metabolism.

Subcellular location: Membrane; Multi-pass membrane protein.

Sequence similarities: Belongs to the neutral sphingomyelinase family.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000