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B4GALT4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645832

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
B4GALT4, Polyclonal Antibody
Full Product Name
B4GALT4 (Beta-1,4-galactosyltransferase 4, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4, N-acetyllactosamine synthase, Nal synthase, Lactotriaosylceramide beta-1,4-galactosyl
Product Gene Name
anti-B4GALT4 antibody
Product Synonym Names
Anti -B4GALT4 (Beta-1,4-galactosyltransferase 4, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4, N-acetyllactosamine synthase, Nal synthase, Lactotriaosylceramide beta-1,4-galactosyl
Immunogen Sequence
MGFNLTFHLS YKFRLLLLLT LCLTVVGWAT SNYFVGAIQE IPKAKEFMAN FHKTLILGKG KTLTNEASTK KVELDNCPSV SPYLRGQSKL IFKPDLTLEE VQAENPKVSR GRYRPEECKA LQRVAILVPH RNREKHLMYL LEHLHPFLQR QQLDYGIYVI HQAEGKKFNR AKLLNVGYLE ALKEENWDCF IFHDVDLVPE NDFNLYKCEE HPKHLVVGRN STGYRLRYSG YFGGVTALSR EQFFKVNGFS NNYWGWGGED DDLRLRVELQ RMKISRPLPE VGKYTMVFHT RDKGNEVNAE RMKLLHQVSR VWRTDGLSSC SYKLVSVEHN PLYINITVDF WFGA
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human B4GALT4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
40,041 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (118930589..118959754, complement). Location: 3q13.3
OMIM
604015
3D Structure
ModBase 3D Structure for O60513
Immunogen
Full length human B4GALT4, aa1-344 (AAH04523.1).

Product Categories/Family for anti-B4GALT4 antibody

Antibodies; Abs to Enzymes; Glycosyltransferase
Applications Tested/Suitable for anti-B4GALT4 antibody
Western Blot (WB)
Application Notes for anti-B4GALT4 antibody
Suitable for use in Western Blot.

Western Blot (WB) of anti-B4GALT4 antibody

B4GALT4 rabbit polyclonal antibody. Western Blot analysis of B4GALT4 expression in human spleen.
Rabbit Anti-B4GALT4 Polyclonal Antibody – Cat# MBS645832

Western Blot (WB) of anti-B4GALT4 antibody

Western Blot analysis of B4GALT4 expression in transfected 293T cell line by B4GALT4 polyclonal antibody. Lane 1: B4GALT4 transfected lysate (40kD). Lane 2: Non-transfected lysate.
Rabbit Anti-B4GALT4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS645832

Other Notes

Small volumes of anti-B4GALT4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for B4GALT4 purchase

MBS645832 is an antibody targeting B4GALT4. Also known as B4GALT4 (Beta-1,4-galactosyltransferase 4, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4, N-acetyllactosamine synthase, Nal synthase, Lactotriaosylceramide beta-1,4-galactosyl. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for B4GALT4. It may not necessarily be applicable to this product.

NCBI GI #
37183134
NCBI GeneID
8702
NCBI Accession #
AAQ89367.1
UniProt Primary Accession #
O60513
UniProt Secondary Accession #
Q68D68; Q9BSW3; Q9C078
UniProt Related Accession #
O60513
NCBI Official Full Name
B4GALT4
NCBI Official Synonym Full Names
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
NCBI Official Symbol
B4GALT4
NCBI Official Synonym Symbols
B4Gal-T4; beta4Gal-T4
UniProt Protein Name
Beta-1,4-galactosyltransferase 4
UniProt Synonym Protein Names
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4Including the following 2 domains:N-acetyllactosamine synthase (EC:2.4.1.90)Alternative name(s):Nal synthase
Protein Family
Beta-1,4-galactosyltransferase
UniProt Gene Name
B4GALT4
UniProt Synonym Gene Names
Beta-1,4-GalTase 4; Beta4Gal-T4; b4Gal-T4
UniProt Entry Name
B4GT4_HUMAN
NCBI Protein Information
beta-1,4-galactosyltransferase 4; beta-1,4-GalTase 4; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4; beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 4; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4

UniProt Comments for B4GALT4

Function: Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Ref.2

Catalytic activity: UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine. Ref.2UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide. Ref.2

Cofactor: Manganese

By similarity.

Pathway: Protein modification; protein glycosylation.

Subcellular location: Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Trans cisternae of Golgi stack.

Tissue specificity: High expression in heart, placenta, kidney and pancreas; lower in brain, colon, lung, muscle, ovary, testis and uterus.

Sequence similarities: Belongs to the glycosyltransferase 7 family.

Biophysicochemical propertiesKinetic parameters:KM=238 µM for GlcNAc-B-S-pNP Ref.3
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-B4GALT4 antibody

 Reference Product  PubMed Publications
 B3GNT5 antibody  >2 publications with B4GALT4 and B3GNT5

Pathways associated with anti-B4GALT4 antibody

 Products by Pathway  Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
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 Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies  Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies  Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram
 Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies  Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram
 Glycosphingolipid Biosynthesis, Neolacto-series, LacCer => NLc4Cer Pathway antibodies  Glycosphingolipid Biosynthesis, Neolacto-series, LacCer => NLc4Cer Pathway Diagram
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 Keratan Sulfate Biosynthesis Pathway antibodies  Keratan Sulfate Biosynthesis Pathway Diagram

Organs/Tissues associated with anti-B4GALT4 antibody

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >1 publications with B4GALT4 and Intestine
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