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PEAK1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645902

Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN. More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PEAK1, Monoclonal Antibody
Full Product Name
PEAK1 (Pseudopodium-enriched Atypical Kinase 1, Sugen Kinase 269, Tyrosine-protein Kinase SgK269, KIAA2002, FLJ21140, FLJ34483)
Product Gene Name
anti-PEAK1 antibody
Product Synonym Names
Anti -PEAK1 (Pseudopodium-enriched Atypical Kinase 1, Sugen Kinase 269, Tyrosine-protein Kinase SgK269, KIAA2002, FLJ21140, FLJ34483)
Immunogen Sequence
TPPPLPKKMI IRANTEPISK DLQKSMESSL CVMANPTYDI DPNWDASSAG SSISYELKGL DIESYDSLER PLRKERPVPS AANSISSLTT LSIKDRFSNS
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3E7
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.4.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Concentration
0.5mg/ml (lot specific)
Specificity
Recognizes human PEAK 1
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
193,106 Da
Chromosome Location
Chromosome: 15; NC_000015.9 (77400498..77712446, complement). Location: 15q24.3
OMIM
614248
3D Structure
ModBase 3D Structure for Q9H792
Immunogen
Partial recombinant corresponding to aa1151-1250 of human PEAK1 with GST tag. MW of GST tag alone is 26kD.

Product Categories/Family for anti-PEAK1 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-PEAK1 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-PEAK1 antibody
Immunofluorescence: 10ug/ml
Optimal dilutions to be determined by the researcher.

Western Blot (WB) of anti-PEAK1 antibody

Western Blot detection against Immunogen (36.63kD).
Mouse Anti-PEAK1 Monoclonal Antibody – Cat# MBS645902

Western Blot (WB) of anti-PEAK1 antibody

Western Blot analysis of PEAK1 expression in MCF-7.
Mouse Anti-PEAK1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS645902

Immunofluorescence (IF) of anti-PEAK1 antibody

Immunofluorescence analysis of PEAK1 in HeLa cells using MBS645902 (10ug/ml).
Mouse Anti-PEAK1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS645902

ELISA (EIA) of anti-PEAK1 antibody

Detection limit for recombinant GST tagged PEAK1 is ~0.3ng/ml using MBS645902 as a capture antibody in sandwich ELISA.
Mouse Anti-PEAK1 Monoclonal Antibody, ELISA (EIA) – Cat# MBS645902

Other Notes

Small volumes of anti-PEAK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PEAK1 purchase

The 190 kD protein Pseudopodium enriched atypical kinase 1 (PEAK1) is a non-receptor tyrosine kinase that interacts with the actin portion of the cytoskeleton. It is believed to play a role in cell spreading, migration, and proliferation through phosphorylation of cytoskeleton- associated proteins. PEAK1 plays a pivotal role in regulating the formation of focal adhesions during cell migration. PEAK1 acts downstream of integrin and EGFR signaling and is itself tyrosine phosphorylated via the Src pathway. Altering the PEAK1 levels in cancer cells increases the anchorage-independent cell growth and causes tumor progression in mice. As PEAK1 levels are elevated in human colon cancer and pancreatic cancer cells, this protein is a possible target for cancer therapy.
This affinity purified mouse anti human PEAK1 monoclonal antibody is suitable for ELISA, western blot, and immunofluorescence applications. The anti PEAK1 antibody is specific for human PEAK1 and shows no species crossreactivity.

Additional Details

NCBI/Uniprot data below describe general gene information for PEAK1. It may not necessarily be applicable to this product.

NCBI GI #
148368962
NCBI GeneID
79834
NCBI Accession #
NP_079052.2
UniProt Primary Accession #
Q9H792
UniProt Secondary Accession #
Q6ZS78; Q8NAZ4; Q8NCM3; Q8TEG7
UniProt Related Accession #
Q9H792
GenBank Nucleotide #
NM_024776.3
NCBI Official Full Name
pseudopodium-enriched atypical kinase 1
NCBI Official Symbol
PEAK1
NCBI Official Synonym Symbols
SGK269
UniProt Protein Name
Pseudopodium-enriched atypical kinase 1
UniProt Synonym Protein Names
Sugen kinase 269; Tyrosine-protein kinase SgK269
Protein Family
Pseudopodium-enriched atypical kinase
UniProt Gene Name
PEAK1
UniProt Synonym Gene Names
KIAA2002
UniProt Entry Name
PEAK1_HUMAN
NCBI Protein Information
pseudopodium-enriched atypical kinase 1; sugen kinase 269; tyrosine-protein kinase SgK269; pseudopodium enriched atypical kinase

NCBI Summary for PEAK1

This gene encodes a non-receptor tyrosine kinase that is a member of the new kinase family three (NFK3) family. In migrating cells, the encoded protein is associated with the actin cytoskeleton and focal adhesions and promotes developing focal adhesion elongation. This protein may play a role in the regulation of cell migration, proliferation and cancer metastasis. [provided by RefSeq, Mar 2014]
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UniProt Comments for PEAK1

Function: Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN. Ref.7

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.7

Subunit structure: Interacts with BCAR1 and CRK. Ref.7

Subcellular location: Cytoplasm › cytoskeleton. Cell junction › focal adhesion. Note: Colocalizes with F-actin in serum-rich medium. Actin colocalization is reduced during serum starvation. Ref.7

Post-translational modification: Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Autophosphorylated in vitro. Ref.7

Sequence similarities: Belongs to the protein kinase superfamily.Contains 1 protein kinase domain.

Sequence caution: The sequence BAB15006.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC87076.1 differs from that shown. Reason: Erroneous initiation.
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Research Articles on PEAK1

1. results define a novel signaling pathway in basal breast cancer involving Lyn and SgK269 that offers clinical opportunities for therapeutic intervention

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PEAK1 antibody

 Reference Product  PubMed Publications
 GRB2 antibody  >2 publications with PEAK1 and GRB2
 CRK antibody  >1 publications with PEAK1 and CRK

Diseases associated with anti-PEAK1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >5 publications with PEAK1 and Neoplasms
 Scleroderma, Diffuse Antibodies  >1 publications with PEAK1 and Scleroderma, Diffuse
 Breast Neoplasms Antibodies  >1 publications with PEAK1 and Breast Neoplasms
 Liver Neoplasms Antibodies  >1 publications with PEAK1 and Liver Neoplasms
 Lymphoma Antibodies  >1 publications with PEAK1 and Lymphoma

Organs/Tissues associated with anti-PEAK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3 publications with PEAK1 and Blood
 Liver Antibodies  >1 publications with PEAK1 and Liver
 Muscle Antibodies  >1 publications with PEAK1 and Muscle
 Brain Antibodies  >1 publications with PEAK1 and Brain
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