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ACER3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6463201

MBS6463201 is an antibody targeting ACER3. Also known as ACER3, CT (Alkaline Dihydroceramidase SB89, Alkaline Phytoceramidase, aPHC, Alkaline Ceramidase 3, Alkaline CDase 3, AlkCDase 3, APHC, PHCA) (Azide free) (HRP). Reactive in Human, Mouse. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ACER3, Polyclonal Antibody
Full Product Name
ACER3, CT (Alkaline Dihydroceramidase SB89, Alkaline Phytoceramidase, aPHC, Alkaline Ceramidase 3, Alkaline CDase 3, AlkCDase 3, APHC, PHCA) (Azide free) (HRP)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human ACER3. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
31,552 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (76571917..76734850). Location: 11q13.5
3D Structure
ModBase 3D Structure for Q9NUN7
Immunogen
Synthetic peptide selected from the C-terminal region of human ACER3 (KLH).
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-ACER3 antibody

Applications Tested/Suitable for anti-ACER3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-ACER3 antibody
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ACER3 antibody

Western blot analysis of ACER3 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). ACER3 (arrow) was detected using the purified Pab.
Rabbit Anti-ACER3 Polyclonal Antibody – Cat# MBS6463201

Flow Cytometry (FC/FACS) of anti-ACER3 antibody

ACER3 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ACER3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6463201

Product References and Citations for anti-ACER3 antibody

1. Wheeler, H.E., et.al., PLoS Genet. 5(10), E1000685 (2009). 2. Mao, C. et.al., Biochim. Biophys. Acta 1781(9), 424-434 (2008).

Other Notes

Small volumes of anti-ACER3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ACER3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alkaline ceramidase 3
NCBI Official Synonym Full Names
alkaline ceramidase 3
NCBI Official Symbol
ACER3
NCBI Official Synonym Symbols
APHC; PHCA
UniProt Protein Name
Alkaline ceramidase 3
UniProt Synonym Protein Names
Alkaline dihydroceramidase SB89; Alkaline phytoceramidase
Protein Family
UniProt Gene Name
ACER3
UniProt Synonym Gene Names
APHC; PHCA; AlkCDase 3; Alkaline CDase 3; aPHC
UniProt Entry Name
ACER3_HUMAN
NCBI Protein Information
alkaline ceramidase 3; alkCDase 3; alkaline CDase 3; alkaline phytoceramidase; phytoceramidase, alkaline; alkaline dihydroceramidase SB89

UniProt Comments for ACER3

ACER3: Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity. Belongs to the alkaline ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; EC 3.5.1.-; Hydrolase; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; integral to Golgi membrane

Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; positive regulation of cell proliferation; sphingosine biosynthetic process; ceramide metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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