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ASIP Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6464254

MBS6464254 is an antibody targeting ASIP. Also known as ASIP, CT (Atypical PKC Isotype-specific-interacting Protein, Bazooka, Baz, CTCL Tumor Antigen se2-5, FLJ21015, Partitioning Defective 3 Homolog, PAR3, PAR-3, PARD3, PARD-3, PAR3alpha, PAR3-alpha, PAR3A,… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ASIP, Polyclonal Antibody
Full Product Name
ASIP, CT (Atypical PKC Isotype-specific-interacting Protein, Bazooka, Baz, CTCL Tumor Antigen se2-5, FLJ21015, Partitioning Defective 3 Homolog, PAR3, PAR-3, PARD3, PARD-3, PAR3alpha, PAR3-alpha, PAR3A, PARD3A, SE2-5L16, SE2-5LT1, SE2-5T2) (APC)
Product Gene Name
anti-ASIP antibody
Product Synonym Names
Par-3 Partitioning Defective 3 Homolog (C. elegans)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human PARD3. Species Crossreactivity: mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
149,695 Da
OMIM
606745
3D Structure
ModBase 3D Structure for Q8TEW0
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1329-1356 in the C-terminal region of human PARD3, UniProt Accession #Q8TEW0. Species sequence homology: rat
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-ASIP antibody

Antibodies; Adapter Proteins
Applications Tested/Suitable for anti-ASIP antibody
Western Blot (WB), FLISA
Application Notes for anti-ASIP antibody
WB: 1:100-1:500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ASIP antibody

Western Blot analysis of MBS626297 in mouse stomach tissue lysates (35ug/lane). PARD3 (arrow) was detected using the purified Pab.
Rabbit Anti-ASIP Polyclonal Antibody – Cat# MBS6464254

Immunohistochemistry (IHC) of anti-ASIP antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted withMBS626297, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Rabbit Anti-ASIP Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6464254

Flow Cytometry (FC/FACS) of anti-ASIP antibody

Flow cytometric analysis of k562 cells using MBS626297 (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ASIP Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6464254

Product References and Citations for anti-ASIP antibody

1. Noda, Y., et.al., Genes Cells 6(2), 107-119 (2001). 2. Beausoleil, S.A., et.al., Proc. Natl. Acad. Sci. USA 101(33), 12130-12135 (2004).

Other Notes

Small volumes of anti-ASIP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ASIP. It may not necessarily be applicable to this product.

NCBI GI #
21361831
NCBI GeneID
56288
NCBI Accession #
NP_062565.2
UniProt Primary Accession #
Q8TEW0
UniProt Secondary Accession #
Q5T2U1; Q5VUA2; Q5VUA3; Q5VWV0; Q5VWV1; Q5VWV3; Q5VWV4; Q5VWV5; Q6IQ47; Q8TCZ9; F5H5T0
UniProt Related Accession #
Q8TEW0
GenBank Nucleotide #
NM_019619.3
NCBI Official Full Name
partitioning defective 3 homolog isoform 1
NCBI Official Synonym Full Names
par-3 family cell polarity regulator
NCBI Official Symbol
PARD3
NCBI Official Synonym Symbols
Baz; ASIP; PAR3; PARD-3; PARD3A; SE2-5T2; PPP1R118; SE2-5L16; SE2-5LT1; PAR3alpha
UniProt Protein Name
Partitioning defective 3 homolog
UniProt Synonym Protein Names
Atypical PKC isotype-specific-interacting protein; ASIP; CTCL tumor antigen se2-5; PAR3-alpha
Protein Family
Agouti-signaling protein
UniProt Gene Name
PARD3
UniProt Synonym Gene Names
PAR3; PAR3A; PAR-3; PARD-3; ASIP
UniProt Entry Name
PARD3_HUMAN
NCBI Protein Information
partitioning defective 3 homolog; bazooka; PAR3-alpha; CTCL tumor antigen se2-5; par-3 partitioning defective 3 homolog; par-3 family cell polarity regulator alpha; protein phosphatase 1, regulatory subunit 118; atypical PKC isotype-specific interacting p

NCBI Summary for ASIP

This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for ASIP

PAR3-alpha: an adapter protein involved in asymmetrical cell division, and cell polarization processes. Polarity complex proteins, including PARD3, are essential for PIP3-induced receptor tyrosine kinase recycling. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Association with PARD6B may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons. PDZ 1 domain modulates interactions with JAM1, PARD6A and PARD6B; PDZ 2 domain mediates interaction with membranes containing phosphoinositol lipids; PDZ 3 domain regulates interactions with PTEN (via C-terminus). Belongs to the PAR3 family. 10 isoforms of the human protein are produced by alternative splicing. Isoform 2, but not at least isoform 3 interacts with PRKCZ.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p11.21

Cellular Component: internode region of axon; cell-cell adherens junction; protein complex; tight junction; cell soma; apical part of cell; plasma membrane; endomembrane system; spindle; intercellular junction; cell cortex; cytosol; cell junction

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; protein kinase C binding; protein phosphatase binding; phosphatidylinositol 3-phosphate binding

Biological Process: positive regulation of myelination; intercellular junction assembly and maintenance; regulation of actin filament-based process; establishment and/or maintenance of cell polarity; microtubule cytoskeleton organization and biogenesis; cell cycle; myelination in the peripheral nervous system; centrosome localization; axonogenesis; asymmetric cell division; transforming growth factor beta receptor signaling pathway; protein kinase C activation; protein complex assembly; protein targeting to membrane
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Research Articles on ASIP

1. A cytoplasmic expression of PAR-3 is therefore implicated in worse clinical and pathological cancer features in clear cell renal cell carcinoma and could be useful to identify patients with high-risk tumors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ASIP antibody

 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Cell-cell Junction Organization Pathway antibodies  Cell-cell Junction Organization Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram

Diseases associated with anti-ASIP antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >12 publications with ASIP and Carcinoma
 Nervous System Diseases Antibodies  >7 publications with ASIP and Nervous System Diseases
 Cell Transformation, Neoplastic Antibodies  >7 publications with ASIP and Cell Transformation, Neoplastic
 Congenital Abnormalities Antibodies  >6 publications with ASIP and Congenital Abnormalities
 Adenocarcinoma Antibodies  >5 publications with ASIP and Adenocarcinoma
 Kidney Diseases Antibodies  >5 publications with ASIP and Kidney Diseases
 Liver Neoplasms Antibodies  >4 publications with ASIP and Liver Neoplasms
 Inflammation Antibodies  >4 publications with ASIP and Inflammation
 Necrosis Antibodies  >3 publications with ASIP and Necrosis
 Liver Diseases Antibodies  >2 publications with ASIP and Liver Diseases

Organs/Tissues associated with anti-ASIP antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >24 publications with ASIP and Brain
 Kidney Antibodies  >21 publications with ASIP and Kidney
 Blood Antibodies  >16 publications with ASIP and Blood
 Eye Antibodies  >14 publications with ASIP and Eye
 Vascular Antibodies  >12 publications with ASIP and Vascular
 Nerve Antibodies  >11 publications with ASIP and Nerve
 Liver Antibodies  >8 publications with ASIP and Liver
 Embryonic Tissue Antibodies  >7 publications with ASIP and Embryonic Tissue
 Skin Antibodies  >7 publications with ASIP and Skin
 Mammary Gland Antibodies  >6 publications with ASIP and Mammary Gland
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