• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CENPK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647482

CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CENPK, Polyclonal Antibody
Full Product Name
CENPK, CT (CENPK, ICEN37, Centromere protein K, Interphase centromere complex protein 37, Protein AF-5alpha, p33)
Product Gene Name
anti-CENPK antibody
Product Synonym Names
Anti -CENPK, CT (CENPK, ICEN37, Centromere protein K, Interphase centromere complex protein 37, Protein AF-5alpha, p33)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
31,655 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (64813593..64858995, complement). Location: 5q12.3
OMIM
611502
3D Structure
ModBase 3D Structure for Q9BS16
Immunogen
CENPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CENPK.

Product Categories/Family for anti-CENPK antibody

Antibodies; Abs to Centromeres
Applications Tested/Suitable for anti-CENPK antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CENPK antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Western Blot (WB) of anti-CENPK antibody

Western blot analysis of CENPK Antibody (C-term) in mouse liver tissue lysates (35ug/lane). CENPK (arrow) was detected using the purified Pab.
Rabbit Anti-CENPK Polyclonal Antibody – Cat# MBS647482

Immunohistochemistry (IHC) of anti-CENPK antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with CENPK Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-CENPK Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS647482

Other Notes

Small volumes of anti-CENPK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CENPK purchase

MBS647482 is an antibody targeting CENPK. Also known as CENPK, CT (CENPK, ICEN37, Centromere protein K, Interphase centromere complex protein 37, Protein AF-5alpha, p33). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CENPK. It may not necessarily be applicable to this product.

NCBI GI #
388490144
NCBI GeneID
64105
NCBI Accession #
NP_001253967.1
UniProt Primary Accession #
Q9BS16
UniProt Secondary Accession #
Q9H4L0
UniProt Related Accession #
Q9BS16
GenBank Nucleotide #
NM_001267038.1
NCBI Official Full Name
centromere protein K
NCBI Official Synonym Full Names
centromere protein K
NCBI Official Symbol
CENPK
NCBI Official Synonym Symbols
P33; Solt; CENP-K; FKSG14; AF5alpha
UniProt Protein Name
Centromere protein K
UniProt Synonym Protein Names
Interphase centromere complex protein 37; Protein AF-5alpha; p33
Protein Family
Centromere protein
UniProt Gene Name
CENPK
UniProt Synonym Gene Names
ICEN37; CENP-K
UniProt Entry Name
CENPK_HUMAN
NCBI Protein Information
centromere protein K; protein AF-5alpha; leucine zipper protein FKSG14; SoxLZ/Sox6-binding protein Solt; interphase centromere complex protein 37

NCBI Summary for CENPK

CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM, Mar 2008]

UniProt Comments for CENPK

Function: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore. Ref.5 Ref.6 Ref.8

Subunit structure: Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts directly with CENPH. Ref.6 Ref.7 Ref.9

Subcellular location: Nucleus. Chromosome › centromere. Chromosome › centromere › kinetochore. Note: Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex. Ref.5 Ref.6

Tissue specificity: Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta. Ref.1

Involvement in disease: Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with KMT2A/MLL1.

Sequence similarities: Belongs to the CENPK family.
Show more

Research Articles on CENPK

1. the vertebrate KNL1 counterpart is essential for chromosome segregation and is required to localize a subset of outer kinetochore proteins.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CENPK antibody

 Reference Product  PubMed Publications
 CDCA8 antibody  >1 publications with CENPK and CDCA8
 NDC80 antibody  >1 publications with CENPK and NDC80
 CENPH antibody  >1 publications with CENPK and CENPH

Pathways associated with anti-CENPK antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway antibodies  Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram
 Mitotic Metaphase And Anaphase Pathway antibodies  Mitotic Metaphase And Anaphase Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
 Nucleosome Assembly Pathway antibodies  Nucleosome Assembly Pathway Diagram
 Resolution Of Sister Chromatid Cohesion Pathway antibodies  Resolution Of Sister Chromatid Cohesion Pathway Diagram

Diseases associated with anti-CENPK antibody

 Disease Name  Pubmed Publications
 Hepatitis Antibodies  >1 publications with CENPK and Hepatitis
 Neoplasms Antibodies  >1 publications with CENPK and Neoplasms

Organs/Tissues associated with anti-CENPK antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with CENPK and Blood
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000