Goat Anti-AGAP3 Polyclonal Antibody – Cat# MBS647653

(5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

CENTG3 (AGAP3) Antibody – Goat Polyclonal

Name: AGAP3 Antibody; Polyclonal; Goat; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS647653
Price: 835 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS647653

CENTG3, or M-RIP, is a 116kD protein that interacts with myosin phosphatase and RhoA in vascular smooth muscle cells. Because CENTG3 is able to assemble a complex containing both RhoA and MBS, it is speculated that this protein may play a role in myosin… More details

$835 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CENTG3 (AGAP3), Polyclonal Antibody

Full Product Name

CENTG3 (Arf-GAP with GTPase, ANK Repeat and PH Domain-containing Protein 3, AGAP-3, CRAM-associated GTPase, CRAG, Centaurin-gamma-3, Cnt-g3, MR1-interacting Protein, MRIP-1, AGAP3)

Product Gene Name

anti-AGAP3 antibody

Product Synonym Names

Anti -CENTG3 (Arf-GAP with GTPase, ANK Repeat and PH Domain-containing Protein 3, AGAP-3, CRAM-associated GTPase, CRAG, Centaurin-gamma-3, Cnt-g3, MR1-interacting Protein, MRIP-1, AGAP3)

Clonality

Polyclonal

Host

Goat

Species Reactivity

Human, Mouse, Rat

Form/Format

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human AGAP3. Species sequence homology: Mouse and rat.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

95,044 Da

Chromosome Location

Chromosome: 7; NC_000007.13 (150782918..150841523). Location: 7q36.1

3D Structure

ModBase 3D Structure for Q96P47

Immunogen

Synthetic peptide corresponding to C-NPSAELHRSPSLL, from human AGAP3, at C-terminal (NP_114152.3).

Product Categories/Family for anti-AGAP3 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

Applications Tested/Suitable for anti-AGAP3 antibody

ELISA (EL/EIA), Immunohistochemistry (IHC)

Application Notes for anti-AGAP3 antibody

Suitable for use in ELISA and Immunohistochemistry.
Dilution: ELISA: 1:32,000
Immunohistochemistry (Paraffin): 5-10ug/ml

Other Notes

Small volumes of anti-AGAP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AGAP3 purchase

MBS647653 is an antibody targeting AGAP3. Also known as CENTG3 (Arf-GAP with GTPase, ANK Repeat and PH Domain-containing Protein 3, AGAP-3, CRAM-associated GTPase, CRAG, Centaurin-gamma-3, Cnt-g3, MR1-interacting Protein, MRIP-1, AGAP3). Reactive in Human, Mouse, Rat. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for AGAP3. It may not necessarily be applicable to this product.

NCBI GI #

119574414

NCBI GeneID

116988

NCBI Accession #

EAW54029.1

UniProt Primary Accession #

Q96P47

UniProt Secondary Accession #

Q59EN0; Q96RK3

UniProt Related Accession #

Q96P47

NCBI Official Full Name

centaurin, gamma 3, isoform CRA_b

NCBI Official Synonym Full Names

ArfGAP with GTPase domain, ankyrin repeat and PH domain 3

NCBI Official Symbol

AGAP3

NCBI Official Synonym Symbols

CRAG; AGAP-3; CENTG3; MRIP-1; cnt-g3

UniProt Protein Name

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3

UniProt Synonym Protein Names

CRAM-associated GTPase; CRAG; Centaurin-gamma-3; Cnt-g3; MR1-interacting protein

Protein Family

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein

UniProt Gene Name

AGAP3

UniProt Synonym Gene Names

CENTG3; AGAP-3; CRAG; Cnt-g3; MRIP-1

UniProt Entry Name

AGAP3_HUMAN

NCBI Protein Information

arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3; centaurin-gamma-3; centaurin, gamma 3; CRAM-associated GTPase; MR1-interacting protein; CRMP (collapsin response mediator protein) associated

UniProt Comments for AGAP3

Function: GTPase-activating protein for the ADP ribosylation factor family

Potential. GTPase which may be involved in the degradation of expanded polyglutamine proteins through the ubiquitin-proteasome pathway. Ref.6

Enzyme regulation: GTPase activity is stimulated by oxidative stress. Ref.6

Subunit structure: Interacts with PML. Interacts with expanded polyglutamine proteins. Ref.6

Subcellular location: Cytoplasm. Note: In cells upon oxidative stress or in brains of Machado-Joseph disease patients, translocates to PML nuclear bodies. Ref.6

Tissue specificity: Widely expressed. Ref.5 Ref.6

Sequence similarities: Belongs to the centaurin gamma-like family.Contains 3 ANK repeats.Contains 1 Arf-GAP domain.Contains 1 PH domain.

Research Articles on AGAP3

1. M-RIP can assemble a complex containing both RhoA and Myosin phosphatase myosin binding subunit, suggesting that M-RIP may play a role in myosin phosphatase regulation by RhoA

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AGAP3 antibody

Reference Product	PubMed Publications
FOS antibody	>1 publications with AGAP3 and FOS

Pathways associated with anti-AGAP3 antibody

Products by Pathway	Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram

Organs/Tissues associated with anti-AGAP3 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2 publications with AGAP3 and Brain