PIN4 Antibody – Rabbit Polyclonal

Function: Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA. Ref.10Isoform 2 binds to double-stranded DNA. Ref.10

Catalytic activity: Peptidylproline (omega=180) = peptidylproline (omega=0).

Subunit structure: Isoform 1 is found in pre-ribosomal ribonucleoprotein (pre-rRNP) complexes

By similarity. Ref.10

Subcellular location: Isoform 1: Nucleus › nucleolus. Cytoplasm › cytoskeleton › spindle. Cytoplasm. Note: Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1. Ref.1 Ref.2 Ref.6 Ref.7 Ref.9 Ref.10Isoform 2: Mitochondrion. Mitochondrion matrix. Note: Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide. Ref.1 Ref.2 Ref.6 Ref.7 Ref.9 Ref.10

Tissue specificity: Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed. Ref.1 Ref.2 Ref.5

Domain: The PPIase domain enhances mitochondrial targeting.

Post-translational modification: Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus. Ref.7 Ref.10Isoform 2 is sumoylated with SUMO2 and SUMO3. Ref.5

Sequence similarities: Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily.Contains 1 PpiC domain.

Sequence caution: The sequence AAH05234.2 differs from that shown. Reason: Erroneous initiation. The sequence AAH70288.1 differs from that shown. Reason: Erroneous initiation. The sequence AAI04654.1 differs from that shown. Reason: Erroneous initiation. The sequence AAI11395.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAG54532.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.