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atp1b2a Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648563

The protein belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ATP1B2 (atp1b2a), Polyclonal Antibody
Full Product Name
ATP1B2, ID (ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Sodium/potassium-dependent ATPase subunit beta-2)
Product Gene Name
anti-atp1b2a antibody
Product Synonym Names
Anti -ATP1B2, ID (ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Sodium/potassium-dependent ATPase subunit beta-2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Concentration
0.57 mg/ml (lot specific)
Specificity
Recognizes human ATP1B2
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
Chromosome: 23; NC_007134.5 (45574170..45605616, complement). Location: chromosome: 23
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 115-141 in the central region of human ATP1B2. Species sequence homology: bovine, mouse, rat

Product Categories/Family for anti-atp1b2a antibody

Antibodies; Abs to Ion Channel
Applications Tested/Suitable for anti-atp1b2a antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-atp1b2a antibody
Dilution:

Western Blot: 1:1000
Immunohistochemistry: 1:50-1:100
Flow Cytometry: 1:10-50

Optimal dilutions should be determined by the researcher

Western Blot (WB) of anti-atp1b2a antibody

Western blot analysis of ATP1B2 Antibody (Center) in K562 cell line lysates (35ug/lane). ATP1B2 (arrow) was detected using the purified Pab.
Rabbit Anti-atp1b2a Polyclonal Antibody – Cat# MBS648563

Immunohistochemistry (IHC) of anti-atp1b2a antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATP1B2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-atp1b2a Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS648563

Flow Cytometry (FC/FACS) of anti-atp1b2a antibody

ATP1B2 Antibody (Center) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-atp1b2a Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS648563

Western Blot (WB) of anti-atp1b2a antibody

Western Blot analysis of lysates from k562 cell line and rat liver tissue lysate (35ug/lane, from left to right) using MBS648563 (1:1000/lane). IgG H&L (HRP) goat anti-rabbit (1:10,000) was used as the secondary antibody
Rabbit Anti-atp1b2a Polyclonal Antibody, Western Blot (WB) – Cat# MBS648563

Other Notes

Small volumes of anti-atp1b2a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for atp1b2a purchase

MBS648563 is an antibody targeting atp1b2a. Also known as ATP1B2, ID (ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Sodium/potassium-dependent ATPase subunit beta-2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for atp1b2a. It may not necessarily be applicable to this product.

NCBI GI #
18858315
NCBI GeneID
64269
NCBI Accession #
NP_571744.1
GenBank Nucleotide #
NM_131669.3
NCBI Official Full Name
ATPase, Na+/K+ transporting, beta 2a polypeptide
NCBI Official Synonym Full Names
ATPase, Na+/K+ transporting, beta 2a polypeptide
NCBI Official Symbol
atp1b2a
NCBI Official Synonym Symbols
cb711; atp1b2; zgc:86580; wu:fc09e11
NCBI Protein Information
ATPase, Na+/K+ transporting, beta 2a polypeptide; ATPase, Na+/K+ transporting, beta 2a polypeptide; ATPase, Na+/K+ beta 2 polypeptide; ATPase, Na+/K+ transporting, beta 2 polypeptide

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-atp1b2a antibody

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 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Ion Channel Transport Pathway antibodies  Ion Channel Transport Pathway Diagram
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 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
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