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ACTN3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649355

Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ACTN3, Polyclonal Antibody
Full Product Name
ACTN3, ID (ACTN3, Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein)
Product Gene Name
anti-ACTN3 antibody
Product Synonym Names
Anti -ACTN3, ID (ACTN3, Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
103,241 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (66314312..66330799). Location: 11q13.1
OMIM
102574
3D Structure
ModBase 3D Structure for Q08043
Immunogen
ACTN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-437 amino acids from the Central region of human ACTN3.

Product Categories/Family for anti-ACTN3 antibody

Antibodies; Abs to Actin Related Proteins
Applications Tested/Suitable for anti-ACTN3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ACTN3 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Western Blot (WB) of anti-ACTN3 antibody

ACTN3 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the ACTN3 antibody detected the ACTN3 protein (arrow).
Rabbit Anti-ACTN3 Polyclonal Antibody – Cat# MBS649355

Immunohistochemistry (IHC) of anti-ACTN3 antibody

ACTN3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTN3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-ACTN3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649355

Other Notes

Small volumes of anti-ACTN3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTN3 purchase

MBS649355 is an antibody targeting ACTN3. Also known as ACTN3, ID (ACTN3, Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTN3. It may not necessarily be applicable to this product.

NCBI GI #
385648244
NCBI GeneID
89
NCBI Accession #
NP_001245300.1
UniProt Primary Accession #
Q08043
UniProt Secondary Accession #
Q4KKV2; A6NP77
UniProt Related Accession #
Q08043
GenBank Nucleotide #
NM_001258371.1
NCBI Official Full Name
alpha-actinin-3 isoform 2
NCBI Official Synonym Full Names
actinin, alpha 3
NCBI Official Symbol
ACTN3
UniProt Protein Name
Alpha-actinin-3
UniProt Synonym Protein Names
Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein
Protein Family
Alpha-actinin
UniProt Gene Name
ACTN3
UniProt Entry Name
ACTN3_HUMAN
NCBI Protein Information
alpha-actinin-3; F-actin cross-linking protein; alpha-actinin skeletal muscle

NCBI Summary for ACTN3

This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants. The reference genome contains the non-coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Apr 2012]
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UniProt Comments for ACTN3

Function: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Subunit structure: Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1. Ref.5

Tissue specificity: Expressed only in a subset of type 2 skeletal muscle fibers. Ref.4

Polymorphism: About 18% of the world population lack a functional ACTN3 due to a stop codon polymorphism at position 577. The absence of a functional ACTN3 expression is not correlated with a disease state [

MIM:102574].

Sequence similarities: Belongs to the alpha-actinin family.Contains 1 actin-binding domain.Contains 2 CH (calponin-homology) domains.Contains 2 EF-hand domains.Contains 4 spectrin repeats.

Sequence caution: The sequence AP002748 differs from that shown. Reason: According to the human genome assembly there is a stop codon in position 577 which is only found in 18% of the world population.
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Research Articles on ACTN3

1. results provide more solid evidence for the associations between ACE II genotype and endurance events and between ACTN3 R allele and power events

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ACTN3 antibody

 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram

Diseases associated with anti-ACTN3 antibody

 Disease Name  Pubmed Publications
 Atrophy Antibodies  >4 publications with ACTN3 and Atrophy
 Neoplasms Antibodies  >3 publications with ACTN3 and Neoplasms
 Kidney Diseases Antibodies  >2 publications with ACTN3 and Kidney Diseases
 Gonadal Disorders Antibodies  >1 publications with ACTN3 and Gonadal Disorders
 Ovarian Diseases Antibodies  >1 publications with ACTN3 and Ovarian Diseases
 Ovarian Neoplasms Antibodies  >1 publications with ACTN3 and Ovarian Neoplasms

Organs/Tissues associated with anti-ACTN3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >98 publications with ACTN3 and Muscle
 Heart Antibodies  >5 publications with ACTN3 and Heart
 Bone Antibodies  >3 publications with ACTN3 and Bone
 Embryonic Tissue Antibodies  >3 publications with ACTN3 and Embryonic Tissue
 Eye Antibodies  >1 publications with ACTN3 and Eye
 Testis Antibodies  >1 publications with ACTN3 and Testis
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