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RAD54L Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649767

The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a… More details

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Product Overview

Online Datasheet in PDF
Product Name
RAD54 (RAD54L), Polyclonal Antibody
Full Product Name
RAD54, CT (RAD54L, RAD54A, DNA repair and recombination protein RAD54-like, RAD54 homolog)
Product Gene Name
Product Synonym Names
Anti -RAD54, CT (RAD54L, RAD54A, DNA repair and recombination protein RAD54-like, RAD54 homolog)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
84,352 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (46713367..46744145). Location: 1p32
OMIM
603615
3D Structure
ModBase 3D Structure for Q92698
Immunogen
RAD54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 641-671 amino acids from the C-terminal region of human RAD54.
Applications Tested/Suitable for anti-RAD54L antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-RAD54L antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-RAD54L antibody

Western Blot analysis of NCI-H460 cell line lysates (35ug/lane) using MBS649767. This demonstrates that MBS649767 detects the RAD54 protein (arrow).
Rabbit Anti-RAD54L Polyclonal Antibody – Cat# MBS649767

Flow Cytometry (FC/FACS) of anti-RAD54L antibody

Flow cytometric analysis of NCI-H460 cell using MBS649767 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-RAD54L Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS649767

Other Notes

Small volumes of anti-RAD54L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RAD54L purchase

MBS649767 is an antibody targeting RAD54L. Also known as RAD54, CT (RAD54L, RAD54A, DNA repair and recombination protein RAD54-like, RAD54 homolog). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RAD54L. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
RAD54
NCBI Official Synonym Full Names
RAD54-like (S. cerevisiae)
NCBI Official Symbol
RAD54L
NCBI Official Synonym Symbols
HR54; hHR54; RAD54A; hRAD54
UniProt Protein Name
DNA repair and recombination protein RAD54-like
UniProt Synonym Protein Names
RAD54 homolog
UniProt Gene Name
RAD54L
UniProt Synonym Gene Names
RAD54A; hHR54; hRAD54
UniProt Entry Name
RAD54_HUMAN
NCBI Protein Information
DNA repair and recombination protein RAD54-like; RAD54 homolog

UniProt Comments for RAD54L

Function: Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments

By similarity. May play also an essential role in telomere length maintenance and telomere capping in mammalian cells. Ref.7 Ref.9 Ref.10

Subunit structure: Interacts (via N-terminus) with RAD51. Ref.6

Subcellular location: Nucleus Ref.1.

Induction: Expression increases approximately 3-fold in late G1 phase compared to other phases of the cell cycle. Ref.1

Sequence similarities: Belongs to the SNF2/RAD54 helicase family.Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RAD54L antibody

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