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EIF4ENIF1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649907

Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism. More details

$785 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EIF4ENIF1, Polyclonal Antibody
Full Product Name
EIF4ENIF1, CT (Eukaryotic Translation Initiation Factor 4E Transporter, 4E-T, eIF4E Transporter, Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1)
Product Gene Name
Product Synonym Names
Anti -EIF4ENIF1, CT (Eukaryotic Translation Initiation Factor 4E Transporter, 4E-T, eIF4E Transporter, Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1)
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human
Form/Format
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human EIF4ENIF1. Species sequence homology: mouse and rat
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
108,201 Da
Chromosome Location
Chromosome: 22; NC_000022.10 (31835345..31885936, complement). Location: 22q11.2
OMIM
607445
3D Structure
ModBase 3D Structure for Q9NRA8
Immunogen
Synthetic peptide corresponding to C-AKVISVDELEYRQ from human EIF4ENIF1 at C-terminal.
Applications Tested/Suitable for anti-EIF4ENIF1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-EIF4ENIF1 antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 0.25-0.5ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml

Immunohistochemistry (IHC) of anti-EIF4ENIF1 antibody

Human Pancreas (formalin-fixed, paraffin-embedded) stained with EIF4ENIF1 antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.
Goat Anti-EIF4ENIF1 Polyclonal Antibody – Cat# MBS649907

Western Blot (WB) of anti-EIF4ENIF1 antibody

Antibody staining (0.25ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-EIF4ENIF1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS649907

Other Notes

Small volumes of anti-EIF4ENIF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF4ENIF1 purchase

MBS649907 is an antibody targeting EIF4ENIF1. Also known as EIF4ENIF1, CT (Eukaryotic Translation Initiation Factor 4E Transporter, 4E-T, eIF4E Transporter, Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1). Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF4ENIF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
EIF4ENIF1 protein, partial
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E nuclear import factor 1
NCBI Official Symbol
EIF4ENIF1
NCBI Official Synonym Symbols
4E-T; Clast4
UniProt Protein Name
Eukaryotic translation initiation factor 4E transporter
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 4E nuclear import factor 1
UniProt Gene Name
EIF4ENIF1
UniProt Synonym Gene Names
4E-T
UniProt Entry Name
4ET_HUMAN
NCBI Protein Information
eukaryotic translation initiation factor 4E transporter; 2610509L04Rik; eIF4E transporter; eIF4E-transporter

NCBI Summary for EIF4ENIF1

The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
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UniProt Comments for EIF4ENIF1

Function: Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.

Subunit structure: Interacts with EIF4E. Interacts with importin beta only in the presence of importin alpha, suggesting a direct interaction with importin alpha. Interacts with APOBEC3G in an RNA-dependent manner. Ref.7

Subcellular location: Cytoplasm. Nucleus. Nucleus › PML body. Nucleus speckle. Note: Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Localization to nuclear foci and speckles requires active transcription. Ref.16

Tissue specificity: Widely expressed.

Sequence caution: The sequence BAB15092.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC11194.1 differs from that shown. Reason: Erroneous initiation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EIF4ENIF1 antibody

 Reference Product  PubMed Publications
 EIF4E antibody  >14 publications with EIF4ENIF1 and EIF4E

Organs/Tissues associated with anti-EIF4ENIF1 antibody

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