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Fibroblast Growth Factor, Basic, Bovine Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650532

Single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and… More details

$750 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Fibroblast Growth Factor, Basic, Bovine, Active Protein
Full Product Name
Fibroblast Growth Factor, Basic, Recombinant, Bovine (FGFb)
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Al a-Gly-Ser.
Form/Format
Supplied as a lyophilized powder from 1% HSA. Reconstitute in sterile ddH2O to a concentration of >0.1mg/ml.
Purity/Purification
Highly Purified
97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
17.25kD
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
rbFGF-b is fully biologically active when compared to standards. The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be less then 0.1ng/ml, corresponding to a specific activity of 3x10e6 Units/mg.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a standard solution of FGF-b as a Reference Standard.

Product Categories/Family for Fibroblast Growth Factor, Basic, Bovine active protein

Growth Factors; Cytokines; Growth Factors-FGF

Other Notes

Small volumes of Fibroblast Growth Factor, Basic, Bovine active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Fibroblast Growth Factor, Basic, Bovine purchase

MBS650532 is an active protein targeting Fibroblast Growth Factor, Basic, Bovine. Also known as Fibroblast Growth Factor, Basic, Recombinant, Bovine (FGFb).
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