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DMGDH Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9605450

Function: Catalyzes the demethylation of N, N-dimethylglycine to sarcosine. Also has activity with sarcosine in vitro.Subunit Structure: Monomer.Similarity: Belongs to the GcvT family. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DMGDH, Polyclonal Antibody
Full Product Name
DMGDH Antibody
Product Gene Name
Product Synonym Names
Dimethylglycine dehydrogenase; Dimethylglycine dehydrogenase, mitochondrial; Dmgdh; M2GD_HUMAN; ME2GLYDH; mitochondrial
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
DMGDH antibody detects endogenous levels of total DMGDH
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
DMGDH peptide (MBS9624167) is used for blocking the activity of DMGDH antibody (MBS9605450)
Molecular Weight
Observed: 97 kD
Predicted: 97 kDa
3D Structure
ModBase 3D Structure for Q9UI17
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide
Subcellular Location
Mitochondrion.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep
Similarity
Pig (100%), Bovine (86%), Horse (90%), Sheep (90%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-DMGDH antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-DMGDH antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500

Western Blot (WB) of anti-DMGDH antibody

Western blot analysis of extracts from HT-29 cells using DMGDH antibody.The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-DMGDH Polyclonal Antibody – Cat# MBS9605450

Immunofluorescene (IF) of anti-DMGDH antibody

MBS9605450 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-DMGDH Polyclonal Antibody, Immunofluorescene (IF) – Cat# MBS9605450

Western Blot (WB) of anti-DMGDH antibody

Western blot analysis of extracts from HT-29 cells using DMGDH antibody.The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-DMGDH Polyclonal Antibody, Western Blot (WB) – Cat# MBS9605450

Other Notes

Small volumes of anti-DMGDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DMGDH purchase

MBS9605450 is an antibody targeting DMGDH. Also known as DMGDH Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DMGDH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
dimethylglycine dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
dimethylglycine dehydrogenase
NCBI Official Symbol
DMGDH
NCBI Official Synonym Symbols
DMGDHD; ME2GLYDH
UniProt Protein Name
Dimethylglycine dehydrogenase, mitochondrial
UniProt Synonym Protein Names
ME2GLYDH
UniProt Gene Name
DMGDH
NCBI Protein Information
dimethylglycine dehydrogenase, mitochondrial

NCBI Summary for DMGDH

This gene encodes an enzyme involved in the catabolism of choline, catalyzing the oxidative demethylation of dimethylglycine to form sarcosine. The enzyme is found as a monomer in the mitochondrial matrix, and uses flavin adenine dinucleotide and folate as cofactors. Mutation in this gene causes dimethylglycine dehydrogenase deficiency, characterized by a fishlike body odor, chronic muscle fatigue, and elevated levels of the muscle form of creatine kinase in serum. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
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UniProt Comments for DMGDH

Catalyzes the demethylation of N,N-dimethylglycine to sarcosine. Also has activity with sarcosine in vitro.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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