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HpyF3I (DdeI)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6506759

HpyF3I (DdeI) restriction enzyme recognizes C^TNAG sites and cuts best at 37°C in Tango buffer. Includes universal Tango buffer for double-digestions More details

$445 / 500 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HpyF3I (DdeI), Biochemical
Full Product Name
HpyF3I (DdeI)
Host
E Coli that carries the cloned hpyF3IR gene from Helicobacter pylori RFL3 RFL1
Concentration
~10U/ul (lot specific)
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Isoschizomers
BstDEI, DdeI
Methylation Sensitivity
Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive
Enzyme
HpyF3I (DdeI)
Compatible Buffer
10x Buffer Tango
Sensitive to Heat Inactivation
Yes
Optimal Reaction Temperature
37 degree C
Type IIS RE
No
Unit Definition
One unit is defined as the amount of HpyF3I required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of recommended reaction buffer.
Storage Buffer
HpyF3I is supplied in: 10mM Tris-HCl, (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
HpyF3I is inactivated by incubation at 65 degree C for 20 min.
Application Notes for HpyF3I (DdeI) biochemical
Molecular cloning; Restriction site mapping; Genotyping; Southern Blot; Restriction fragment length polymorphism (RFLP); SNP analysis

Other Notes

Small volumes of HpyF3I (DdeI) biochemical vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HpyF3I (DdeI) purchase

MBS6506759 is a biochemical targeting HpyF3I (DdeI). Host: E Coli that carries the cloned hpyF3IR gene from Helicobacter pylori RFL3 RFL1.
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