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Sec61A1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6506892

Sec61 translocon is a channel complex located on the endoplasmic reticulum (ER) membrane to mediate membrane protein insertion into the organelle (1). There are three components in the complex, Sec61A, Sec61B and Sec61G (2). Sec61A is the main component of… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Sec61A1 (HSEC61, Protein Transport Protein SEC61 alpha Subunit Isoform 1), Monoclonal Antibody
Full Product Name
Sec61A1 (HSEC61, Protein Transport Protein SEC61 alpha Subunit Isoform 1)
Product Gene Name
anti-Sec61A1 antibody
Product Synonym Names
Sec61A1
Clonality
Monoclonal
Isotype
IgG
Clone Number
D7Q6V
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Purity/Purification
Purified
Specificity
Recognizes endogenous human Sec61A1. Species Crossreactivity: mouse and rat
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
38,849 Da
OMIM
609213
3D Structure
ModBase 3D Structure for P61619
Immunogen
Synthetic peptide corresponding to residues surrounding Arg273 of human Sec61A1.

Product Categories/Family for anti-Sec61A1 antibody

Antibodies-Primary; Antibodies-Transport Proteins
Applications Tested/Suitable for anti-Sec61A1 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-Sec61A1 antibody
Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:100

Western Blot (WB) of anti-Sec61A1 antibody

Western blot analysis of extracts from various cell lines using Sec61A1 Rabbit mAb.
Rabbit Anti-Sec61A1 Monoclonal Antibody – Cat# MBS6506892

Western Blot (WB) of anti-Sec61A1 antibody

Immunoprecipitation of Sec61A1 protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit mAb IgG Isotype Control, and lane 3 is Sec61A1 Rabbit mAb. Western blot analysis was performed using Sec61A1 Rabbit mAb.
Rabbit Anti-Sec61A1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6506892

Other Notes

Small volumes of anti-Sec61A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Sec61A1 purchase

MBS6506892 is an antibody targeting Sec61A1. Also known as Sec61A1 (HSEC61, Protein Transport Protein SEC61 alpha Subunit Isoform 1). Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Sec61A1. It may not necessarily be applicable to this product.

NCBI GI #
7019415
NCBI GeneID
29927
NCBI Accession #
NP_037468.1
UniProt Primary Accession #
P61619
UniProt Secondary Accession #
P38378; P57726; Q5JPF8; Q8N0Z4; Q8N3U3; Q8NC71; Q9BU16; Q9Y2R3
UniProt Related Accession #
P61619
GenBank Nucleotide #
NM_013336.3
NCBI Official Full Name
protein transport protein Sec61 subunit alpha isoform 1
NCBI Official Synonym Full Names
Sec61 alpha 1 subunit (S. cerevisiae)
NCBI Official Symbol
SEC61A1
NCBI Official Synonym Symbols
SEC61; HSEC61; SEC61A
UniProt Protein Name
Protein transport protein Sec61 subunit alpha isoform 1
Protein Family
Protein transport protein
UniProt Gene Name
SEC61A1
UniProt Synonym Gene Names
SEC61A; Sec61 alpha-1
UniProt Entry Name
S61A1_HUMAN
NCBI Protein Information
protein transport protein Sec61 subunit alpha isoform 1; Sec61 alpha-1; protein transport protein SEC61 alpha subunit; sec61 homolog

NCBI Summary for Sec61A1

The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq, Jul 2008]
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UniProt Comments for Sec61A1

SEC61A1: Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane- bound ribosomes, either directly or through adapter proteins. Belongs to the SecY/SEC61-alpha family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Endoplasmic reticulum; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: rough endoplasmic reticulum; membrane; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; ribosome binding

Biological Process: endoplasmic reticulum organization and biogenesis; SRP-dependent cotranslational protein targeting to membrane; protein targeting to ER; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; posttranslational protein targeting to membrane; cell growth
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Research Articles on Sec61A1

1. Short secretory proteins appear to be ubiquitously transported across the ER membrane through the Sec61 translocon.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Sec61A1 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing-Cross Presentation Pathway antibodies  Antigen Processing-Cross Presentation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 ER-Phagosome Pathway antibodies  ER-Phagosome Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Protein Export Pathway antibodies  Protein Export Pathway Diagram

Diseases associated with anti-Sec61A1 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >4 publications with Sec61A1 and Liver Diseases
 Liver Neoplasms Antibodies  >3 publications with Sec61A1 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >2 publications with Sec61A1 and Carcinoma, Hepatocellular
 Fatty Liver Antibodies  >2 publications with Sec61A1 and Fatty Liver
 Disease Models, Animal Antibodies  >1 publications with Sec61A1 and Disease Models, Animal
 Hyperglycemia Antibodies  >1 publications with Sec61A1 and Hyperglycemia
 Abnormalities, Drug-Induced Antibodies  >1 publications with Sec61A1 and Abnormalities, Drug-Induced
 Prostatic Diseases Antibodies  >1 publications with Sec61A1 and Prostatic Diseases

Organs/Tissues associated with anti-Sec61A1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >4 publications with Sec61A1 and Liver
 Blood Antibodies  >3 publications with Sec61A1 and Blood
 Nerve Antibodies  >2 publications with Sec61A1 and Nerve
 Spleen Antibodies  >2 publications with Sec61A1 and Spleen
 Pituitary Gland Antibodies  >1 publications with Sec61A1 and Pituitary Gland
 Pancreas Antibodies  >1 publications with Sec61A1 and Pancreas
 Bone Marrow Antibodies  >1 publications with Sec61A1 and Bone Marrow
 Bone Antibodies  >1 publications with Sec61A1 and Bone
 Kidney Antibodies  >1 publications with Sec61A1 and Kidney
 Brain Antibodies  >1 publications with Sec61A1 and Brain
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