RPMI 1640 Medium w/o L-Glutamine, Calcium Free Culture Media

Powder

Cell Culture Grade

Store powdered media in Room Temperature. Opened bottles should be capped tighly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Orange, homogeneous, free flowing powder

Magenta, clear, complete

8.7

< 1EU/mL

Dissolve 10.04g in 900ml of ddH20, stirring until completely solubilized. Do NOT heat. Add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22um membrane. Aliquot into sterile containers.

Note: It may be necessary to lower the pH to 4.0 with 1 N HCI to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.

Inorganic Salts
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.4
Sodium Chloride: 6
Sodium Phosphate Dibasic: 0.8

Amino Acids:
L-ArginineHCI: 0.242
L-Asparagine•H20: 0.0568
L-Aspartic Acid: 0.02
L-Cystine•2HCI: 0.0652
L-Glutamic Acid: 0.02
Glycine: 0.01
L-Histidine•HCI•H20: 0.020
Hydroxy-L -pro line: 0.02
L –Isoleucine: 0.05
L-Leucine: 0.05
L-Lysine•HCI: 0.04
L-Methionine: 0.015
L-Phenylalanine: 0.015
L-Proline: 0.02
L-Serine: 0.03
L-Threonine: 0.02
L-Tryptophan: 0.005
L-Tyrosine•HCI: 0.024
L-Valine: 0.02

Vitamins:
p-Aminobenzoic Acid: 0.001
D-Biotin: 0.0002
Choline Chloride: 0.003
Cyanocobalamin: 0.000005
Folic Acid: 0.001
myo-Inositol: 0.035
Niacinamide: 0.001
D-Pantothenic Acid, Na: 0.00025
Pyridoxine•HCI: 0.001
Riboflavin: 0.0002
Thiamine•HCl: 0.001

Other:
D-Glucose: 2.0
Glutathione: 0.001
Phenol Red, Sodium: 0.005

Total:
10.04g/Liter

Culture Media; Media-Cell Culture; RPMI