Nutrient Mixture Ham's F-12 w/L-Glutamine w/o Phenol Red Culture Media

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

<1EU/ml

Light pink , homogeneous, free flowing powder

Light pink, clear, complete

Dissolve 10.70g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 1.18g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

8.2

Inorganic Salts:
Calcium Chloride•2H2O: 0.0441
Cupric Sulfate•5H2O: 0.0000025
Ferrous Sulfate•7H2O: 0.000834
Magnesium Chloride•6H2O: 0.123
Potassium Chloride: 0.224
Sodium Chloride: 7.599
Sodium Phosphate Dibasic: 0.14204
Zinc Sulfate•7H2O: 0.000863

Amino Acids:
L-Alanine: 0.009
L-Arginine•HCl: 0.211
L-Asparagine•H2O: 0.01501
L-Aspartic Acid: 0.0133
L-Cysteine•HCL•H2O: 0.035
L-Glutamic Acid: 0.0147
L-Glutamine: 0.146
Glycine: 0.00751
L-Histidine•HCl•H2O: 0.02096
L-Isoleucine: 0.00394
L-Leucine: 0.0131
L-Lysine•HCl: 0.0365
L-Methionine: 0.00448
L-Phenylalanine: 0.00496
L-Proline: 0.0345
L-Serine: 0.0105
L-Threonine: 0.0119
L-Tryptophan: 0.00204
L-Tyrosine•Na2•2H2O: 0.00778
L-Valine: 0.0117

Vitamins:
D-Biotin: 0.0000073
Choline Chloride: 0.01396
Vitamin B-12: 0.00136
Folic Acid: 0.00132
myo-Inositol: 0.018
Niacinamide: 0.000037
D-Pantothenic Acid•Ca: 0.00048
Pyridoxine•HCl: 0.000062
Riboflavin: 0.000038
Thiamine•HCl: 0.00034

Other:
Thymidine: 0.00073
D-Glucose: 1.802
Hypoxanthine: 0.00408
Linoleic Acid: 0.000084
Thioctic Acid: 0.00021
Putrescine•2HCl: 0.000161
Pyruvic Acid, Sodium: 0.11
Phenol Red, Sodium: Absent

Total: 10.701g/liter

Culture Media; Media-Cell Culture; Nutrient Mixture Ham's