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Ribonuclease H (Sta7437_3452) Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653783

Ribonuclease H specifically degrades only the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded or double-stranded DNA and RNA. More details

$830 / 100 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ribonuclease H (Sta7437_3452), Enzyme
Full Product Name
Ribonuclease H (RNase H)
Product Gene Name
Host
E Coli MRE-600 cells
Form/Format
Supplied as a liquid in 25mM HEPES-KOH, pH 8.0, 50mM KCl, 1mM DTT, 1mM EDTA, 0.1mg/ml BSA, and 50% glycerol.
Purity/Purification
Molecular Biology Grade
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
19kD
Chromosome Location
NC_019748.1 (3903609..3904082, complement). Location: isolation source: pool at botanical garden, country: Cuba: Havana, other: 23.1333 N 82.3667 W
CAS Number
9050-76-4
Unit Definition
One unit catalyzes the formation of 1nmol of acid soluble products in 20min at 37ºC. Enzyme activity is assayed in the following mixture: 20mM Tris-HCl (H 7.8), 40 mM KCl, 8mM MgCl2, 1mM DTT, 24uM [3H]-POLY(A)-POLY(dT), 0.03mg/ml BSA AND 4% Glycerol.

Product Categories/Family for Sta7437_3452 enzyme

Application Notes for Sta7437_3452 enzyme
Suitable for use in Removal of mRNA prior to the synthesis of the 2nd strand of cDNA, Removal of mRNA poly(A) sequences after hybridization with oligo(dT), Site-specific cleavage of RNA and Studies of in vitro polyadenylation rection products.

Other Notes

Small volumes of Sta7437_3452 enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Sta7437_3452 purchase

MBS653783 is an enzyme targeting Sta7437_3452. Also known as Ribonuclease H (RNase H). Host: E Coli MRE-600 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for Sta7437_3452. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Official Full Name
Ribonuclease H
NCBI Official Symbol
Sta7437_3452
Protein Family
NCBI Protein Information
Ribonuclease H

UniProt Comments for Sta7437_3452

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Sta7437_3452 enzyme

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