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Zymolyase 20t Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653884

MBS653884 is an enzyme targeting Zymolyase 20T. Also known as Zymolyase 20T (Lyticase, Yeast Lytic Enzyme). More details

$310 / 100 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Zymolyase 20T, Enzyme
Full Product Name
Zymolyase 20T (Lyticase, Yeast Lytic Enzyme)
Form/Format
Lyophilized powder
Preparation and Storage
Stable for 1 year at 4 degree C. About 70% of the lytic activity is lost when stored at 30 degree C for 3 months.
Activity
21.6 u/mg
Grade
Molecular Biology Grade
Source
Arthrobactor luteus
CAS Number
37340-57-1
Essential Enzyme
B-1, 3-glucan laminaripentaohydrolase
RNase/DNase
Trace Levels
B-1,3-glucannase
1.5 x 10e6 units/g
Protease
1.0 x 10e4 units/g
Mannanase
1.0 x 10e6 units/g.
Optimum pH
7.5 @35 degree C For lysis of viable yeast cells
6.5 @ 45 degree C For hydrolysis of yeast glucan
Stable pH
5-10
Note
Zymolyase 100T is less soluble than 20T and may not be completely dissolved in buffers. If this is the case use as a suspension.
Storage Buffer
0.06M Phosphate Buffer, pH 7.5
Heat Stability
Lytic activity lost on incubation at 60 degree C for 5 minutes.
Activators
SH compounds-2-mercaptoethanol, cysteine or dithiothreitol.
RNase Activity Assay
Trace RNase activity was detected.
Method: MBS653884 was suspended in 50mM Tris-HCl buffer, pH7.5. 10ul of MBS653884 was mixed with 140ul of cCMP (0.1 mg/ml in 50mM Tris-HCl buffer, pH 7.5) and the increase of absorbance at 284nm was measured.
Unit Definition:
One RNase A unit is defined as the amount required for hydrolysis of cCMP that results in an increase of 0.001 absorbance units at 284nm per minute
Unit Definition
One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800nm (A800). Refer to Assay for Enzymatic Activity.
DNase Activity Assay
Trace DNase activity was detected.
Method: MBS653884 was suspended in 50mM Tris-HCl buffer, pH7.5, at a concentration of 2mg/ml and centrifuged to remove the debris. The supernantant was used for the Dnase assay. Reaction mixture (50mM Tris-HCl, pH 7.5, 1mM MnCl2, 50ng/ul lambda DNA-Hindlll-digest, 0.4mg/ml MBS653884) was incubated at 30 degree C for 12 hours, inactivated by heating, and the degradation of lambda DNA was detected by using agarose gel electrophoresis.
Experimental Precautions
Avoid nitrocellulose filters due to non-specific adsorption onto the membrane.

Product Categories/Family for Zymolyase 20T enzyme

Molecular Biology; MB-Enzymes
Application Notes for Zymolyase 20T enzyme
Protoplast/Spheroplast Preparation, Yeast Cell Fusion, Yeast Cell Transformation

Testing Data of Zymolyase 20T enzyme

The extracellular domain is required for Sln1 kinase activation by wall perturbations. A, representative field showing localization of SLN1-GFP (pSS1881) or sln1ΔECD-GFP (pSS1904). BF, bright field. B, Western analysis showing reduced levels of sln1ΔECD compared with full-length SLN1. SLN1, JF2007 (sln1Δ) carrying full-length SLN1-myc (pCLM994); sln1ΔECD, JF2007 carrying sln1ΔECD-myc (pCLM1774). C, comparison of Hog1 phosphorylation kinetics in SLN1 (JF2008 (sln1Δ sho1Δ) carrying full-length (pCLM994)) and sln1ΔECD (JF2008 (sln1Δ sho1Δ) carrying sln1ΔECD (pCLM1774)) strains following addition of 0.4 M NaCl. D, Northern (RNA) hybridization analysis of RNA prepared from wild type
Enzyme – Cat# MBS653884

Testing Data of Zymolyase 20T enzyme

Cultures from the sin 1 (sln1Δ) strain JF2007, or sin 1 (sln1Δ) ccw 12 (sln1Δ), strain JF2368 transformed with the SLN 1 expression plasmids, pCLM994 (SLN1) or pCLM1774 (sln1 (sln1Δ) ECD), and treated with 1 unit/ml zymolyase (+) where indicated.
NCA3 expression was normalized to expression of the SLN1-SKN7-independent CDC33 gene. The extent of induction by zymolyase is shown relative to the untreated SLN1 or sln 1(sln1Δ)ECD strains
Enzyme, Testing Data – Cat# MBS653884

Testing Data of Zymolyase 20T enzyme

Source:
Arthrobacter luteus
Enzyme, Testing Data – Cat# MBS653884

Other Notes

Small volumes of Zymolyase 20T enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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