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CD9 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666226

Information: Human CD9 is strongly expressed on platelets, on early B cells and on activated T cells. CD9 is a member of the tetraspan family crossing the membrane four times. Both the N-terminal and C-terminal regions are on the cytoplasmic side of the… More details

$645 / 120 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD9, Monoclonal Antibody
Full Product Name
anti-human CD9-R-PE
Product Gene Name
anti-CD9 antibody
Product Synonym Names
Monoclonal anti-human CD9/R-PE
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
SN4/C3-3A2
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
Preparation and Storage
Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Molecular Weight
25,416 Da
OMIM
143030
Performance
Five x 105 cultured Nalm-6 human tumor cells were washed and incubated 45 minutes on ice with 80 mul of anti-CD9/R-PE at a 1:50 dilution (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.64 log10 fluorescent units when compared to a Mouse IgG1/PE negative control at a similar concentration. Binding was blocked when pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-CD9 antibody.
Conjugation
PE
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant, and conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A565/A280 ratio of 3.16.
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Membrane antigen preparation of NALM-6
Applications Tested/Suitable for anti-CD9 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD9 antibody
1:50 (or use 1.6mul of concentrated stock per 5 x 105-cell test)

Testing Data of anti-CD9 antibody

Mouse Anti-CD9 Monoclonal Antibody – Cat# MBS666226

Product References and Citations for anti-CD9 antibody

Y. Luo, et al, (1989) Cancer Research 49: 706-710. F. Lanza, et al, (1991) J Biol Chem 266: 10638-10645. K. Kuroda, et al, (1995) J Immunol 155: 4427-4436.

Other Notes

Small volumes of anti-CD9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD9 purchase

MBS666226 is an antibody targeting CD9. Also known as anti-human CD9-R-PE. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD9. It may not necessarily be applicable to this product.

NCBI GI #
4502693
NCBI GeneID
928
NCBI Accession #
NP_001760.1
UniProt Secondary Accession #
Q5J7W6; Q96ES4; D3DUQ9
UniProt Related Accession #
P21926
GenBank Nucleotide #
NM_001769.3
NCBI Official Full Name
CD9 antigen
NCBI Official Synonym Full Names
CD9 molecule
NCBI Official Symbol
CD9
NCBI Official Synonym Symbols
MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
UniProt Protein Name
CD9 antigen
UniProt Synonym Protein Names
5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD_antigen: CD9
Protein Family
CD9 antigen
UniProt Gene Name
CD9
UniProt Synonym Gene Names
MIC3; TSPAN29; MRP-1; Tspan-29
UniProt Entry Name
CD9_HUMAN
NCBI Protein Information
CD9 antigen; 5H9 antigen; tetraspanin-29; BA-2/p24 antigen; CD9 antigen (p24); leukocyte antigen MIC3; motility related protein-1; cell growth-inhibiting gene 2 protein

NCBI Summary for CD9

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]
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UniProt Comments for CD9

CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, multi-pass; Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: extracellular space; platelet alpha granule membrane; focal adhesion; integral to plasma membrane; apical plasma membrane; plasma membrane; vesicle; external side of plasma membrane

Molecular Function: integrin binding; protein binding

Biological Process: negative regulation of cell proliferation; platelet activation; platelet degranulation; fusion of sperm to egg plasma membrane; single fertilization; pathogenesis; brain development; blood coagulation; oligodendrocyte development; cell motility; cell adhesion; response to water deprivation; multicellular organism reproduction; paranodal junction assembly
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Research Articles on CD9

1. CD9 and CD63 tetraspanins block HIV-1-induced cell-cell fusion at the transition from hemifusion to pore opening.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD9 antibody

 Reference Product  PubMed Publications
 CD36 antibody  >37 publications with CD9 and CD36
 IGSF8 antibody  >17 publications with CD9 and IGSF8
 PTGFRN antibody  >12 publications with CD9 and PTGFRN
 ITGA6 antibody  >3 publications with CD9 and ITGA6
 ERVWE1 antibody  >1 publications with CD9 and ERVWE1
 SCARB1 antibody  >1 publications with CD9 and SCARB1
 HBEGF antibody  >1 publications with CD9 and HBEGF

Pathways associated with anti-CD9 antibody

 Products by Pathway  Pathway Diagram
 Fertilization Pathway antibodies  Fertilization Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Reproduction Pathway antibodies  Reproduction Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Sperm:Oocyte Membrane Binding Pathway antibodies  Sperm:Oocyte Membrane Binding Pathway Diagram
 A6b1 And A6b4 Integrin Signaling Pathway antibodies  A6b1 And A6b4 Integrin Signaling Pathway Diagram

Diseases associated with anti-CD9 antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >64 publications with CD9 and Neoplasm Metastasis
 Neoplasms, Germ Cell and Embryonal Antibodies  >57 publications with CD9 and Neoplasms, Germ Cell and Embryonal
 Leukemia, Myeloid, Acute Antibodies  >49 publications with CD9 and Leukemia, Myeloid, Acute
 Inflammation Antibodies  >43 publications with CD9 and Inflammation
 Cardiovascular Diseases Antibodies  >43 publications with CD9 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >36 publications with CD9 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >35 publications with CD9 and Lung Neoplasms
 Necrosis Antibodies  >32 publications with CD9 and Necrosis
 Breast Neoplasms Antibodies  >31 publications with CD9 and Breast Neoplasms
 Disease Models, Animal Antibodies  >30 publications with CD9 and Disease Models, Animal

Organs/Tissues associated with anti-CD9 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >456 publications with CD9 and Blood
 Bone Antibodies  >139 publications with CD9 and Bone
 Bone Marrow Antibodies  >124 publications with CD9 and Bone Marrow
 Vascular Antibodies  >89 publications with CD9 and Vascular
 Lung Antibodies  >81 publications with CD9 and Lung
 Embryonic Tissue Antibodies  >64 publications with CD9 and Embryonic Tissue
 Lymph Node Antibodies  >47 publications with CD9 and Lymph Node
 Placenta Antibodies  >42 publications with CD9 and Placenta
 Kidney Antibodies  >40 publications with CD9 and Kidney
 Liver Antibodies  >38 publications with CD9 and Liver
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