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CD2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666297

Information: Human CD2 is a T-lineage glycoprotein with roles in cell-cell adhesion and the transduction of activation signals. Multiple ligands for CD2 include CD58 (LFA-3), CD59, and CD48. CD2 may play a role in regulating T cell anergy. Antibody UMCD2… More details

$530 / 120 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD2, Monoclonal Antibody
Full Product Name
anti-human CD2-R-PE
Product Gene Name
Product Synonym Names
Monoclonal anti-human CD2(LFA-2, T11)/R-PE
Clonality
Monoclonal
Isotype
IgG2ak
Clone Number
UMCD2/IE7E8
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
Preparation and Storage
Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Performance
Five x 105 cultured human HPB-MLT cells were incubated 45 minutes on ice with 80 ul of product at a dilution factor of 1:50 (5 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.57 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD2 antibody.
Conjugation
PE
Production
Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 250 ug/ml with an A565/A280 ratio of 3.28.
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Human rheumatoid synovial T cell line (ST-1)
Applications Tested/Suitable for anti-CD2 antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-CD2 antibody
1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)

Testing Data of anti-CD2 antibody

Mouse Anti-CD2 Monoclonal Antibody – Cat# MBS666297

Product References and Citations for anti-CD2 antibody

K.F. Kozarsky, et al, (1993) Cell Immunol 150: 235-246. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 342-352. G.M. Bell & J.B. Imboden (1995) J Immunol 155: 2805-2807.

Other Notes

Small volumes of anti-CD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD2 purchase

MBS666297 is an antibody targeting CD2. Also known as anti-human CD2-R-PE. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD2 molecule
NCBI Official Symbol
CD2
NCBI Protein Information
CD2 molecule; cluster of differentiation 2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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