Mouse Anti-CD274 Monoclonal Antibody – Cat# MBS666449

CD274 Antibody – Mouse Monoclonal

Name: CD274 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS666449
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666449

Information: CD274 (B7-H1, PD-L1, Programmed Death Ligand) is a member of the B7 family and is expressed on a variety of tissues including lymphoid cells. It plays an important role in regulation of T cell activation, and is involved in progression of cancer,… More details

$605 / 120 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD274, Monoclonal Antibody

Full Product Name

anti-human CD274(B7-H1)-R-PE

Product Gene Name

anti-CD274 antibody

Product Synonym Names

Monoclonal anti-human CD274 (B7-H1, PD-L1)/R-PE

Clonality

Monoclonal

Isotype

IgG1k

Clone Number

ANC6H1

Host

Mouse

Species Reactivity

Human

Form/Format

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative)

Concentration

Volume: 0.2 ml (lot specific)

Preparation and Storage

Store at 2 - 5°C. Do not freeze! Protect from light.

OMIM

605402

Performance

Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 ug/ml PHA. Five x 105 cells per tube were washed and pre incubated 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-human CD274 at a dilution factor of 1:50 (20 ug/ml). They were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD274 antibody.

Conjugation

Production

Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 1 mg/ml with an A565/A280 ratio of 3.04.

Buffer

50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Immunogen

Recombinant human CD274

Applications Tested/Suitable for anti-CD274 antibody

Flow Cytometry (FC/FACS), ELISA (EIA)

Application Notes for anti-CD274 antibody

1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)

Product References and Citations for anti-CD274 antibody

1)Cancer Research (April 2006) 66:3381. 2)J Molecular Medicine (Feb 2004) 81(5):281. 3)Int J Hematol (Nov 2003) 78(4):321.

Other Notes

Small volumes of anti-CD274 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD274 purchase

MBS666449 is an antibody targeting CD274. Also known as anti-human CD274(B7-H1)-R-PE. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD274. It may not necessarily be applicable to this product.

NCBI GI #

112363645

NCBI GeneID

29126

NCBI Accession #

ABI16084.1

UniProt Secondary Accession #

Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27

UniProt Related Accession #

Q9NZQ7

NCBI Official Full Name

CD274, partial

NCBI Official Synonym Full Names

CD274 molecule

NCBI Official Symbol

CD274

NCBI Official Synonym Symbols

B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

UniProt Protein Name

Programmed cell death 1 ligand 1

UniProt Synonym Protein Names

B7 homolog 1; B7-H1; CD_antigen: CD274

Protein Family

Programmed cell death

UniProt Gene Name

CD274

UniProt Synonym Gene Names

B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1

UniProt Entry Name

PD1L1_HUMAN

NCBI Protein Information

programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1

UniProt Comments for CD274

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: plasma membrane; integral to membrane; endomembrane system; external side of plasma membrane

Molecular Function: protein binding

Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; T cell costimulation; positive regulation of T cell proliferation; immune response; signal transduction

Research Articles on CD274

1. PD-L1 expression on myeloid precursor cells is increased quantitatively and qualitatively in AML patients after gamma-interferon treatment initiation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD274 antibody

Reference Product	PubMed Publications
CD80 antibody	>390 publications with CD274 and CD80
PDCD1 antibody	>322 publications with CD274 and PDCD1
STAT1 antibody	>11 publications with CD274 and STAT1
PTEN antibody	>8 publications with CD274 and PTEN

Pathways associated with anti-CD274 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Costimulation By The CD28 Family Pathway antibodies	Costimulation By The CD28 Family Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
PD-1 Signaling Pathway antibodies	PD-1 Signaling Pathway Diagram

Diseases associated with anti-CD274 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>106 publications with CD274 and Inflammation
Liver Diseases Antibodies	>84 publications with CD274 and Liver Diseases
Necrosis Antibodies	>73 publications with CD274 and Necrosis
Nervous System Diseases Antibodies	>69 publications with CD274 and Nervous System Diseases
Neoplasms, Experimental Antibodies	>65 publications with CD274 and Neoplasms, Experimental
Adenocarcinoma Antibodies	>59 publications with CD274 and Adenocarcinoma
Lung Diseases Antibodies	>44 publications with CD274 and Lung Diseases
Kidney Diseases Antibodies	>43 publications with CD274 and Kidney Diseases
Lung Neoplasms Antibodies	>40 publications with CD274 and Lung Neoplasms
Recurrence Antibodies	>26 publications with CD274 and Recurrence

Organs/Tissues associated with anti-CD274 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>755 publications with CD274 and Blood
Lymph Node Antibodies	>78 publications with CD274 and Lymph Node
Lung Antibodies	>70 publications with CD274 and Lung
Bone Antibodies	>58 publications with CD274 and Bone
Skin Antibodies	>45 publications with CD274 and Skin
Brain Antibodies	>31 publications with CD274 and Brain
Pancreas Antibodies	>28 publications with CD274 and Pancreas
Placenta Antibodies	>19 publications with CD274 and Placenta
Stomach Antibodies	>13 publications with CD274 and Stomach
Mouth Antibodies	>9 publications with CD274 and Mouth