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CD127 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666749

Information: CD127 (IL-7Ra) is the specific receptor component for the cytokine Interleukin -7 (IL-7). It is found on a wide variety of hematopoietic cell types including B cell precursors, and the majority of T cells. Its expression levels are decreased on T… More details

$605 / 120 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD127, Monoclonal Antibody
Full Product Name
anti-human CD127-R-PE
Product Gene Name
Product Synonym Names
Monoclonal anti-human CD127 (IL-7Ra)
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
ANC8F2
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
Concentration
Volume: 0.2 ml (lot specific)
Preparation and Storage
Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Molecular Weight
51,365 Da
3D Structure
ModBase 3D Structure for B9ZSM5
Performance
Five x 105 ficoll prepared human PBMC per tube were pre incubated with 20 ul of 250 ug/ml human Ig (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of product at a dilution factor of 1:50 (10 ug/ml). Cells were washed three times, fixed and analyzed by FACS using a lymphocyte gate. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control. Binding was blocked when the reagent was pre incubated with a 10-fold excess of recombinant CD127-muIg.
Conjugation
PE
Production
Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A565/A280 ratio of 2.64.
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Recombinant human CD127
Applications Tested/Suitable for anti-CD127 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD127 antibody
1:50 (or use 1.6ul of concentrated reagent per 5 x 105-cell test)

Testing Data of anti-CD127 antibody

Mouse Anti-CD127 Monoclonal Antibody – Cat# MBS666749

Product References and Citations for anti-CD127 antibody

1) Noguchi, M. et al., 1993, Science 262:1877. 2) Prieyl, J.A. and T.W. LeBien, 1996, Proc. Natl. Acad. Sci. 93:10348. 3) Goodwin RG, Friend D, Ziegler SF, et al. Cell. 1990;60:941

Other Notes

Small volumes of anti-CD127 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD127 purchase

MBS666749 is an antibody targeting CD127. Also known as anti-human CD127-R-PE. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD127. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
NCBI Official Full Name
CD127 protein
NCBI Official Symbol
IL7R
NCBI Official Synonym Symbols
CD127
UniProt Protein Name
CD127 protein
UniProt Synonym Protein Names
CD127 protein
UniProt Gene Name
CD127
UniProt Entry Name
B9ZSM5_PIG
NCBI Protein Information
interleukin-7 receptor subunit alpha

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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