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IgG/IgM Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666782

Information: Goat anti-Mouse/FITC is reactive with Mouse Ig subclasses: G1, G2a, G2b, G3. It also reacts well with all Mouse IgM antibodies. Reagent does not bind significantly to any human cells or cell lines tested. More details

$315 / 0.5 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgG/IgM, Polyclonal Antibody
Full Product Name
Goat anti-Mouse IgG/IgM-FITC
Product Gene Name
anti-IgG/IgM antibody
Product Synonym Names
Goat anti-Mouse IgG/FITC
Clonality
Polyclonal
Host
Goat
Species Reactivity
Mouse
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Preparation and Storage
Store at 2 - 5 degree C. Freeze/thawing not recommended. Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Cells Tested
Ficolled human peripheral blood lymphocytes, CEM, HPB-MLT, Nalm-6, Jurkat, U-937, Molt-4, Raji, Daudi and KG-1 (Some cells were pre-blocked with human IgG to prevent non specific binding).
Performance
When used as a second step reagent in Flow cytometry, optimal resolution between signal and background with Mouse anti-Human antibodies was obtained using 50 ul of a 1:60 dilution of product to stain 5 x 105 cells.
Conjugation
FITC
Production
Antiserum was obtained from goats immunized with purified mouse IgG, and polyclonal antibody was purified by immunospecific affinity chromatography. Nonspecific binding was reduced by adsorbtion to human serum proteins. The prep was then reacted with FITC. Unconjugated FITC was removed from conjugate using extensive dialysis. Antibody conjugate is at 0.51 mg/ml with a Fluoroscein/Antibody molar ratio of 3.14.
Buffer
10 mM Sodium Phosphate pH 7.5, 150 mM NaCl, 1.0% BSA, 1% Glycerol, 0.1% NaN3 (as a preservative).
Group Header
Goat anti-Mouse IgG/IgM
Immunogen
Mouse IgG
Applications Tested/Suitable for anti-IgG/IgM antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-IgG/IgM antibody
1:60 (Use 50 ul per 5 x 105-cell test)

Other Notes

Small volumes of anti-IgG/IgM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IgG/IgM purchase

MBS666782 is an antibody targeting IgG/IgM. Also known as Goat anti-Mouse IgG/IgM-FITC. Reactive in Mouse. Host: Goat.
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