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UCHL1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS669143

UchL1 (ubiquitin C-terminal hydrolase), also known as PGP9.5 (protein gene product 9.5) and PARK5, is a neuronal biomarker and ubquitin system protein.UchL1 is a highly conserved antibody and has shown that it is expressed in neurons and neuroendocrine cells… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
UchL1, Monoclonal Antibody
Full Product Name
Monoclonal Antibody to UchL1 (Clone: 31A3)
Product Gene Name
Product Synonym Names
UCHL1; Neuron cytoplasmic protein 9.5; PGP9.5; PGP 9.5; Ubiquitin thioesterase L1
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
31A3
Host
Mouse
Species Reactivity
Rat, Mouse, Human
Predicted: Pig
Form/Format
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Purity/Purification
Purified
Protein G Chromatography
Preparation and Storage
Store the antibody at 4 degree C; stable for 6 months.
For long-term storage; store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Molecular Weight
24,824 Da
OMIM
AC095043 Genomic DNA
3D Structure
ModBase 3D Structure for P09936
Antigen
UchL1
Immunogen Information
Native UchL1 (PGP9.5) protein from brain was used as immunogen to generate the antibody.
Conjugation
Unconjugated

Product Categories/Family for anti-UCHL1 antibody

Applications Tested/Suitable for anti-UCHL1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-UCHL1 antibody
WB analysis: 1-3 ug/ml
IHC analysis: 1: 400-1: 800

Western Blot (WB) of anti-UCHL1 antibody

Fig: 1 Western blot analysis analysis of UchL1. Anti-UchL1 antibody (Clone: 31A3) was used at 1 ug/ml in 1) human, 2) mouse and 3) rat brain lysate.
Mouse Anti-UCHL1 Monoclonal Antibody – Cat# MBS669143

Immunohistochemistry (IHC) of anti-UCHL1 antibody

Fig: 2 Immunohistochemical analysis of UchL1 in human Brain using UchL1 antibody (Clone: 31A3) at 1: 500 dilution.
Mouse Anti-UCHL1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS669143

Immunohistochemistry (IHC) of anti-UCHL1 antibody

Fig: 3 Immunohistochemical analysis of UchL1 in human Kidney tissue using UchL1 antibody (Clone: 31A3) at 10 ug/ml.
Mouse Anti-UCHL1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS669143

Other Notes

Small volumes of anti-UCHL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UCHL1 purchase

MBS669143 is an antibody targeting UCHL1. Also known as Monoclonal Antibody to UchL1 (Clone: 31A3). Reactive in Rat, Mouse, Human
Predicted: Pig. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for UCHL1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L1
NCBI Official Synonym Full Names
ubiquitin C-terminal hydrolase L1
NCBI Official Symbol
UCHL1
NCBI Official Synonym Symbols
NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Synonym Protein Names
Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1
UniProt Gene Name
UCHL1
UniProt Synonym Gene Names
UCH-L1; PGP9.5
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1

NCBI Summary for UCHL1

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
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UniProt Comments for UCHL1

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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