Hif1 Alpha ELISA Kit for Human

Background/Introduction: Hypoxia inducible factor-1 (HIF) is a heterodimeric protein including an oxygen-regulated alpha subunit and a constitutive expressed beta subunit. Hypoxia inducible factor-1 alpha (HIF- 1alpha) is a master regulator of cellular adaptation to oxygen deprivation and activates transcription of genes involved in tumor metabolism, angiogenesis, invasion and metastasis, all of which are implicated in cancer progression. Several domains of HIF-1alpha mediate protein-protein interaction, which is essential for the formation of the active heterodimer with HIF-1beta. Targeting specific domains of HIF-1alpha might lead to the identification of more selective inhibitors. Alpha subunits are degraded during normoxia mainly through a proteasomedependent pathway after hydroxylation of two proline residues by prolyl-hydroxilases (PHDs). During hypoxia, PHDs are inhibited and HIF-1alpha subunit accumulates, dimerizes with HIF-1beta and drive expression of HIF target genes which include genes involved in angiogenesis and tissue repair such as vascular endothelial growth factor (VEGF) or erythropoietin (EPO) and prolilhydroxilases (PHDs) genes, among others.

Small volumes of Hif1 Alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS669190 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hif1 Alpha, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Hif1 Alpha. The ELISA analytical biochemical technique of the MBS669190 kit is based on Hif1 Alpha antibody-Hif1 Alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Hif1 Alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Hif1 Alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).