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G-CSF (CSF3) Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670060

MBS670060 is an antibody targeting CSF3. Also known as Rat Anti-Human G-CSF-BIOT. Reactive in Human. Host: Rat. More details

$435 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
G-CSF (CSF3), Monoclonal Antibody
Full Product Name
Rat Anti-Human G-CSF-BIOT
Product Gene Name
anti-CSF3 antibody
Product Synonym Names
Rat Anti-Human G-CSF - Biotin; Rat Anti-Human G-CSF
Clonality
Monoclonal
Isotype
Rat IgG2a kappa
Clone Number
BVD11-37G10
Host
Rat
Species Reactivity
Human
Form/Format
Biotin (BIOT) Conjugate
Specificity
Human Granulocyte-Colony Stimulating Factor (G-CSF)1,2 (This is a neutralizing antibody.)
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
Molecular Weight
22,293 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (38171614..38174066). Location: 17q11.2-q12
OMIM
138970
3D Structure
ModBase 3D Structure for P09919
Immunogen
E. coli-expressed recombinant human G-CSF
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-CSF3 antibody
ELISA; Western Blot
Application Notes for anti-CSF3 antibody
Research Application(s): ELISA Detection: BVD11-37G10 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human G-CSF protein levels.1,2 Biotinylated BVD11-37G10 antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human G-CSF as the standard.
Western Blotting: The purified BVD11-37G10 antibody is also useful for Western blotting applications. A concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant human G-CSF under reducing conditions.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

Product References and Citations for anti-CSF3 antibody

1. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
2. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.

Other Notes

Small volumes of anti-CSF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CSF3. It may not necessarily be applicable to this product.

NCBI GI #
297660573
NCBI GeneID
1440
UniProt Primary Accession #
P09919
UniProt Related Accession #
P09919; Q5FBY7; Q6FH65; Q8N4W3
NCBI Official Full Name
granulocyte colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 3 (granulocyte)
NCBI Official Symbol
CSF3
NCBI Official Synonym Symbols
GCSF; CSF3OS; C17orf33; MGC45931
UniProt Protein Name
Granulocyte colony-stimulating factor
UniProt Synonym Protein Names
Pluripoietin
Protein Family
Granulocyte colony-stimulating factor
UniProt Gene Name
CSF3
UniProt Synonym Gene Names
C17orf33; GCSF
UniProt Entry Name
CSF3_HUMAN
NCBI Protein Information
granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin; OTTHUMP00000164325; OTTHUMP00000164326; OTTHUMP00000164327; OTTHUMP00000164328

NCBI Summary for CSF3

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq]

UniProt Comments for CSF3

Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Post-translational modification: O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

Pharmaceutical use: Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). Used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood).

Sequence similarities: Belongs to the IL-6 superfamily.

Caution: Ref.5 misquotes the gene name as "CSF1".
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Research Articles on CSF3

1. potential biomarker to document the local pre-conception environment for assisted fertilization

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CSF3 antibody

 Reference Product  PubMed Publications
 CSF3R antibody  >8 publications with CSF3 and CSF3R
 ELANE antibody  >1 publications with CSF3 and ELANE

Pathways associated with anti-CSF3 antibody

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram

Diseases associated with anti-CSF3 antibody

 Disease Name  Pubmed Publications
 Hematologic Diseases Antibodies  >8 publications with CSF3 and Hematologic Diseases
 Leukemia Antibodies  >8 publications with CSF3 and Leukemia
 Inflammation Antibodies  >7 publications with CSF3 and Inflammation
 Neoplasms Antibodies  >5 publications with CSF3 and Neoplasms
 Immune System Diseases Antibodies  >5 publications with CSF3 and Immune System Diseases
 Leukemia, Myeloid Antibodies  >4 publications with CSF3 and Leukemia, Myeloid
 Necrosis Antibodies  >4 publications with CSF3 and Necrosis
 Bone Marrow Diseases Antibodies  >4 publications with CSF3 and Bone Marrow Diseases
 Leukemia, Myelogenous, Chronic, BCR-ABL Positive Antibodies  >3 publications with CSF3 and Leukemia, Myelogenous, Chronic, BCR-ABL Positive
 Anemia Antibodies  >2 publications with CSF3 and Anemia

Organs/Tissues associated with anti-CSF3 antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >2 publications with CSF3 and Skin
 Lung Antibodies  >2 publications with CSF3 and Lung
 Brain Antibodies  >1 publications with CSF3 and Brain
 Muscle Antibodies  >1 publications with CSF3 and Muscle
 Uterus Antibodies  >1 publications with CSF3 and Uterus
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