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IL-10 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670083

MBS670083 is an antibody targeting IL-10. Also known as Rat Anti-Mouse IL-10-LE/AF. Reactive in Mouse. Host: Rat. More details

$405 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL-10, Monoclonal Antibody
Full Product Name
Rat Anti-Mouse IL-10-LE/AF
Product Gene Name
anti-IL-10 antibody
Product Synonym Names
Rat Anti-Mouse IL-10 - Low Endotoxin/Azide-Free; Rat Anti-Mouse IL-10
Clonality
Monoclonal
Isotype
Rat IgG2b kappa
Clone Number
JES5-16E3
Host
Rat
Species Reactivity
Mouse
Form/Format
Low Endotoxin, Azide - Free (LE/AF)
Specificity
Mouse Interleukin-10 (IL-10)1, 2 (This is a neutralizing antibody)
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Chromosome Location
NC_004002.1 (2243..2749)
Immunogen
E. coli-expressed recombinant mouse IL-10
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-IL-10 antibody
ELISA; Flow Cytometry; Immunofluorescence
Application Notes for anti-IL-10 antibody
Research Application(s): ELISA Detection: JES5-16E3 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying mouse IL-10 protein levels. Biotinylated JES5-16E3 antibody should be paired with purified JES5-2A5 antibody as the capture antibody, with purified recombinant mouse IL-10 as the standard.
Immunofluorescence/Flow Cytometry: JES6-16E3 antibodies are useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell populations. Recombinant mouse IL-10, unlabeled or biotinylated Rat IgG2b should be used as a specificity control. FITC conjugated JES5-16E3 antibodies are useful for intracellular staining in cells producing IL-10. our intracellular staining buffers are recommended for this application.
Neutralization: The LE/AF form of JES5-16E3 can be used for neutralizing mouse IL-10 bioactivity. A suitable LE/AF Rat IgG2b should be used as an isotype control.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

Product References and Citations for anti-IL-10 antibody

1. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. J. Immunol. Meth. 166:201.
2. Litton, M.J., B. Sander, E. Murphy, and J.S. Abrams. 1994. J. Immunol. Meth. 175:47.

Other Notes

Small volumes of anti-IL-10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-10. It may not necessarily be applicable to this product.

NCBI GI #
21492460
NCBI GeneID
944684
NCBI Accession #
NP_659579.1
GenBank Nucleotide #
NC_004002.1
NCBI Official Full Name
IL-10
NCBI Official Symbol
SPPV_03
NCBI Protein Information
IL-10

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-IL-10 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with IL-10 and Bone
 Connective Tissue Antibodies  >4 publications with IL-10 and Connective Tissue
 Heart Antibodies  >3 publications with IL-10 and Heart
 Muscle Antibodies  >3 publications with IL-10 and Muscle
 Brain Antibodies  >3 publications with IL-10 and Brain
 Vascular Antibodies  >2 publications with IL-10 and Vascular
 Embryonic Tissue Antibodies  >2 publications with IL-10 and Embryonic Tissue
 Ear Antibodies  >1 publications with IL-10 and Ear
 Kidney Antibodies  >1 publications with IL-10 and Kidney
 Umbilical Cord Antibodies  >1 publications with IL-10 and Umbilical Cord
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