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Cd3ec/Cy5.5 Antibody – Hamster Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670248

MBS670248 is an antibody targeting CD3eC/Cy5.5. Also known as Hamster Anti-Mouse CD3e-APC/Cy5.5. Reactive in Mouse. Host: Syrian Hamster. More details

$365 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD3eC/Cy5.5, Monoclonal Antibody
Full Product Name
Hamster Anti-Mouse CD3e-APC/Cy5.5
Product Synonym Names
Hamster Anti-Mouse CD3epsilon
Clonality
Monoclonal
Isotype
IgG, epsilon
Clone Number
500-A2
Host
Syrian Hamster
Species Reactivity
Mouse
Form/Format
Allophycocyanin-Cyanine 5.5 (APC-CY 5.5) Conjugate
Specificity
Mouse CD3epsilon, Mr 25 kDa
CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR).CD3epsilon is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. 500-A2 recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex of mouse strains tested.1, 2, 3
Preparation and Storage
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Immunogen
T cell receptor complexes derived from C6VL-BS thymoma cells (C57BL/Ka)
Characterization
To insure acceptable performance, each batch of product is tested by flow cytometry to conform to the characteristics of a standard reference.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Applications Tested/Suitable for anti-CD3eC/Cy5.5 antibody
Identification and enumeration of CD3+ cells by Flow Cytometry 1, Immunoprecipitation 2, In vitro activation of T cells 2, 3, 4
Application Notes for anti-CD3eC/Cy5.5 antibody
Flow Cytometry: <= 1 ug/10^6 cells

Product References and Citations for anti-CD3eC/Cy5.5 antibody

1. Portoles, P., J. Rojo, A. Golby, M. Bonneville, S. Gromkowski, L. Greenbaum, C. A. Janeway, Jr., D. B. Murphy, and K. Bottomly. 1989. J. Immunol. 142:4169.
2. Allison, J.P., W.L. Havran, M. Poenie, J. Kimura, L. DeGraffenreid, S. Ajami, G. Duwe, A. Weiss, and R. Tsien. 1988. The T-Cell Receptor, UCLA Symposia, 73rd ed. Kappler, J. and M. Davis eds. 33-45.
3. Havran, W.L., M. Poenie, J. Kimura, R. Tsien, A. Weiss, and J.P. Allison. 1987. Nature 330:170-173.
4. Duke, R.C., J.J. Cohen, S.A. Boehme, M.J. Lenardo, C.D. Surh, H. Kishimoto, and J. Sprent. 1995. In: Currents Protocols in Immunology. J. Coligan, A. Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober, eds. John Wiley & Sons, New York, pp. 3.17.1-3.17.33.

Other Notes

Small volumes of anti-CD3eC/Cy5.5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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