CD25 Antibody – Rat Monoclonal

Monoclonal

Rat (Lewis) IgM kappa

7D4

Rat

Mouse

R - phycoerythrin - Cyanine 7 (R - PE - CY7) Conjugate

Low affinity alpha chain IL-2 receptor (IL-2R alpha), Mr 55 kDa
The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.^2 CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.^1, 3, 4 It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220^low TdT^- sIg^- pre-B II and CD45R/B220^low TdT^- sIgM^+sIgD^- immature B cells, but not on the CD45R/B220^low TdT^+ sIg^- pro-B/pre-B I stage nor on CD45R/B220^high TdT^- sIgM^+sIgD^+ mature B cells.^5, 6 CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus.^6, 7 The 7D4 monoclonal antibody reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by the monoclonal antibody 3C7 (Cat. No. 1600-XX). While the monoclonal antibody 7D4 does not block the binding of IL-2 to CD25, when used in combination with the monoclonal antobody 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.^3, 8, 9

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

IL-2-dependent BALB/c mouse helper T-cell clone HT-2

To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.