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CD102 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673727

MBS673727 is an antibody targeting CD102 (ICAM-2). Also known as Rat Anti-Mouse CD102/ICAM-2-PE. Reactive in Mouse. Host: Rat. More details

$340 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD102 (ICAM-2), Monoclonal Antibody
Full Product Name
Rat Anti-Mouse CD102/ICAM-2-PE
Product Synonym Names
Rat Anti-Mouse CD102/ICAM-2 - Phycoerythrin-Labeled; Rat Anti-Mouse CD102 (ICAM-2)
Clonality
Monoclonal
Isotype
Rat (Lewis) IgG2a kappa
Clone Number
3C4
Host
Rat
Species Reactivity
Mouse
Form/Format
R - phycoerythrin (R - PE) Conjugate
Specificity
Mouse ICAM-2
The 3C4 (mIC2/4) monoclonal antibody reacts with mouse ICAM-2 (CD102) cell surface glycoprotein, a ligand for the leucocyte integrin CD11a/CD18 (LFA-1).1 CD102 is constitutively expressed on T cells, B cells, and at high levels on vascular endothelial cells.2 It is also expressed on a variety of leukocyte cell lines.1 The 3C4 antibody blocks the interaction between ICAM-2 and LFA-1.^1
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Immunogen
COS cells transfected with mouse ICAM-2 cDNA
Characterization
To ensure consistency in performance, each batch of product is tested by flow cytometry to conform to the characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-CD102 (ICAM-2) antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
Application Notes for anti-CD102 (ICAM-2) antibody
Research Application(s): In vitro blocking assays

Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells

Product References and Citations for anti-CD102 (ICAM-2) antibody

1. Xu, H., J.K. Bickford, E. Luther, C. Carpenito, F. Takei, and T.A. Springer. 1996. J. Immunol. 156:4909.
2. Xu, H., O.L. Tong, A.R. deFougerolles, and T.A. Springer. 1992. J. Immunol. 149:2650.

Other Notes

Small volumes of anti-CD102 (ICAM-2) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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