Pepsin (PP) ELISA Kit for Human

Name: Human PP ELISA Kit; Pepsin; MyBioSource
Brand: MyBioSource
SKU: MBS042633
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS042633

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only! This kit is intended to be used to determine the level of PP (hereafter termed "analyte") in undiluted original Mouse body fluids and tissue homogenates. This… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Pepsin (PP), ELISA Kit

Full Product Name

Human Pepsin ELISA Kit

Product Gene Name

PP elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Mouse Body Fluids And Tissue Homogenates

Detection Range

0.625ng/ml-20ng/ml

Sensitivity

0.1ng/ml.

Specificity

No significant cross-reactivity or interference between this analyte and analogues is observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%

Molecular Weight

41,993 Da

OMIM

169700

3D Structure

ModBase 3D Structure for P0DJD9

Other Notes

Small volumes of PP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PP purchase

MBS042633 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pepsin (PP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PP. The ELISA analytical biochemical technique of the MBS042633 kit is based on PP antibody-PP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PP. It may not necessarily be applicable to this product.

NCBI GI #

23943854

NCBI GeneID

5222

NCBI Accession #

NP_055039.1

UniProt Primary Accession #

P0DJD9

UniProt Secondary Accession #

P00790; Q7M4R0; Q8N1E3; A8K749; B7ZW62; B7ZW75

UniProt Related Accession #

P0DJD9

GenBank Nucleotide #

NM_014224.3

NCBI Official Full Name

pepsin A-5 preproprotein

NCBI Official Synonym Full Names

pepsinogen 5, group I (pepsinogen A)

NCBI Official Symbol

PGA5

UniProt Protein Name

Pepsin A-5

UniProt Synonym Protein Names

Pepsinogen-5

Protein Family

Pepsin

UniProt Gene Name

PGA5

UniProt Entry Name

PEPA5_HUMAN

NCBI Protein Information

pepsin A-5; pepsinogen-5; pepsinogen A5

NCBI Summary for PP

This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]

UniProt Comments for PP

PGA5: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Belongs to the peptidase A1 family.

Protein type: Secreted; EC 3.4.23.1; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13

Molecular Function: aspartic-type endopeptidase activity

Biological Process: digestion; proteolysis

Research Articles on PP

1. Pepsin and pepsinogen in middle ear effusion are probably caused by LPR and may be involved in the pathogenesis of OME.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PP elisa kit

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