Alpha Beta (V Beta 1) (TCR) Antibody – Mouse Monoclonal

MAb TCR2 precipitates a CD3-associated heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50- kDa upon reduction) on chicken peripheral blood T cells.1 Deglycosylation of the heterodimer yields two polypeptides of Mr 34-kDa and 29-kDa from TCR2 precipitates. In the chicken, two distinct subpopulations of alpha beta T cells appear in the thymus subsequent to the appearance of gamma delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1-3 arise sequentially in the thymus during ontogeny and are now known to represent two distinct V beta families, V beta 1 and V beta 2, respectively.4 The TCR2 MAb reacts with approximately 40% of thymocytes, 40-50% of blood mononuclear cells and 40% of splenocytes in the chicken. Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74+/-2%) or CD8 (26+/-4%). TCR2+ cells in the spleen also express either CD4 (37+/-1%) or CD8 (64+/-4%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2.1-3 This observation led to the demonstration that 71+/-6% of the TCR1(gamma delta)+ cells in the spleen express the CD8 marker.

1. Chen, C.H., J. Cihak, U. Losch, and M.D. Cooper. 1988. Eur. J. Immunol. 18:539.
2. Chen, C.H., J.T. Sowder, J.M. Lahti, J. Cihak, U. Losch, and M.D. Cooper. 1989. Proc. Natl. Acad. Sci. (USA):86:2351
3. Char, D., P. Sanchez, C.H. Chen, R.P. Bucy, and M.D. Cooper. 1990. J. Immunol. 145:3547.
4. Chen, C.H., T.W.F. Gobel, T. Kubota, and M.D. Cooper. 1993. 1994. Poultry Science 73:1012.
5. Sowder, J.T., C.H. Chen, L.L. Ager, J. Cihak, U. Losch, and M.D. Cooper. 1988b. Fed. Proc. 2:874.
6. Cihak, J., U. Losch, G. Hoffman-Hezer, C.H. CHen, M.D. Cooper, and H.W.L. Ziegler-Heitbrock. 1993. Scand. J. Immunol. 38:123.
7. Chen, C.H. 1996. Personal communication.

Small volumes of anti-TCR alpha beta (V beta 1) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS673879 is an antibody targeting TCR alpha beta (V beta 1). Also known as Mouse Anti-Chicken TCRab/Vb1-UNLB. Reactive in Chicken. Host: Mouse.