Mouse Anti-CD44 Monoclonal Antibody – Cat# MBS674136

CD44 Antibody – Mouse Monoclonal

Name: CD44 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS674136
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS674136

MBS674136 is an antibody targeting Cd44. Also known as Mouse Anti-Human CD44-FITC. Reactive in Human. Host: Mouse. More details

$295 / 100 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD44 (Pgp-1), Monoclonal Antibody

Full Product Name

Mouse Anti-Human CD44-FITC

Product Gene Name

anti-Cd44 antibody

Product Synonym Names

Mouse Anti-Human CD44 - FITC; Mouse Anti-Human CD44 (Pgp-1)

Clonality

Monoclonal

Isotype

Mouse IgG2a

Clone Number

F10-44-2

Host

Mouse

Species Reactivity

Human

Form/Format

Fluorescein (FITC) Conjugate

Specificity

Human CD44
CD44, also known as phagocytic glycoprotein-1 (Pgp-1) and the receptor for hyaluronate, exists as a large number of different isoforms resulting from alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells and erythrocytes is a glycosylated type I transmembrane protein. Other variable isoforms containing glycosaminoglycans have molecular weights ranging from 110 to 250 kDa and are widely expressed on hematopoietic and non-hematopoietic cells. CD44 contributes to the adhesion of leukocytes to endothelial cells, stromal cells and the extracellular matrix. It also plays a functional role in cell migration, lymphocyte homing and adhesion during hematopoiesis and lymphocyte activation.1-6

Preparation and Storage

The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE), allophycocyanin (APC), and Spectral Red (SPRD) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

Molecular Weight

85,918 Da

Chromosome Location

Chromosome: 3; NC_005102.2 (88022962..88110352, complement). Location: 3q31

3D Structure

ModBase 3D Structure for O08779

Characterization

To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.

Applications Tested/Suitable for anti-Cd44 antibody

Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation

Application Notes for anti-Cd44 antibody

Flow Cytometry Suggested Dilution: 10 uL/10^6 cells

Product References and Citations for anti-Cd44 antibody

1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.
3. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.
4. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD44 Section, Academic Press, New York, p. 240.
5. Goldstein, L.A., and E.C. Butcher. 1990. Immunogenetics 32:389.
6. Lesley, J., R. Hyman, and P.W. Kincade 1993. Adv. Immunol. 54:271.

Other Notes

Small volumes of anti-Cd44 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Cd44. It may not necessarily be applicable to this product.

NCBI GI #

19923703

NCBI GeneID

25406

NCBI Accession #

NP_037056.2

UniProt Primary Accession #

O08779

UniProt Related Accession #

O08779; O35249; O70509; P26051; Q9EP99; Q9ERN2; Q9ERN3

GenBank Nucleotide #

NM_012924.2

NCBI Official Full Name

CD44 antigen

NCBI Official Synonym Full Names

Cd44 molecule

NCBI Official Symbol

Cd44

NCBI Official Synonym Symbols

CD44A; METAA; RHAMM; MGC124941

UniProt Protein Name

CD44 protein

Protein Family

CD44 antigen

UniProt Gene Name

Cd44

UniProt Synonym Gene Names

CD44

UniProt Entry Name

O08779_RAT

NCBI Protein Information

CD44 antigen; PGP-1; PGP-I; HUTCH-I; ECMR-III; hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; phagocytic glycoprotein I; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; Cell surface glycoprotein CD44 (hyaluronate binding protein)

NCBI Summary for Cd44

adhesion molecule involved in migration, cell fusion and resportion in osteoclasts that also plays a role in cellular metastasis [RGD]

UniProt Comments for Cd44

CD44: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. 19 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: Golgi apparatus; protein complex; focal adhesion; cell surface; basolateral plasma membrane; apical plasma membrane; cytoplasm; plasma membrane; integral to membrane; nucleus; external side of plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; transmembrane receptor activity; cytokine binding; hyaluronic acid binding; phosphoprotein binding; protein kinase binding; epidermal growth factor receptor binding; hyalurononglucosaminidase activity

Biological Process: cell migration; Wnt receptor signaling pathway; positive regulation of heterotypic cell-cell adhesion; positive regulation of neutrophil apoptosis; negative regulation of caspase activity; blood vessel maturation; positive regulation of peptidyl-serine phosphorylation; response to organic cyclic substance; response to vitamin A; hyaluronan catabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of mature B cell apoptosis; ureteric bud branching; cartilage development; regulation of cell growth; inflammatory response; cell adhesion; healing during inflammatory response; neurite development; negative regulation of apoptosis

Research Articles on Cd44

1. Upregulation of hyaluronan and its binding receptors CD44 and LYVE-1 are involved in interstitial fibrosis in chronic cyclosporine-induced renal injury.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Cd44 antibody

Products by Pathway	Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Senescence And Autophagy Pathway antibodies	Senescence And Autophagy Pathway Diagram
TGF-beta Receptor Signaling Pathway antibodies	TGF-beta Receptor Signaling Pathway Diagram
Wnt Signaling Pathway And Pluripotency antibodies	Wnt Signaling Pathway And Pluripotency Diagram

Diseases associated with anti-Cd44 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>3357 publications with Cd44 and Neoplasms
Carcinoma Antibodies	>1355 publications with Cd44 and Carcinoma
Neoplasm Metastasis Antibodies	>803 publications with Cd44 and Neoplasm Metastasis
Skin Diseases Antibodies	>662 publications with Cd44 and Skin Diseases
Inflammation Antibodies	>605 publications with Cd44 and Inflammation
Adenocarcinoma Antibodies	>594 publications with Cd44 and Adenocarcinoma
Breast Neoplasms Antibodies	>502 publications with Cd44 and Breast Neoplasms
Neuroectodermal Tumors Antibodies	>400 publications with Cd44 and Neuroectodermal Tumors
Lung Diseases Antibodies	>363 publications with Cd44 and Lung Diseases
Leukemia Antibodies	>356 publications with Cd44 and Leukemia

Organs/Tissues associated with anti-Cd44 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>579 publications with Cd44 and Lung
Spleen Antibodies	>515 publications with Cd44 and Spleen
Thymus Antibodies	>468 publications with Cd44 and Thymus
Brain Antibodies	>377 publications with Cd44 and Brain
Connective Tissue Antibodies	>261 publications with Cd44 and Connective Tissue
Kidney Antibodies	>241 publications with Cd44 and Kidney
Prostate Antibodies	>227 publications with Cd44 and Prostate
Heart Antibodies	>154 publications with Cd44 and Heart
Eye Antibodies	>137 publications with Cd44 and Eye
Adipose Tissue Antibodies	>111 publications with Cd44 and Adipose Tissue