• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Protein G Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS675013

MBS675013 is an antibody targeting Protein G. Also known as Protein G-UNLB. More details

$325 / 5 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Protein G, Polyclonal Antibody
Full Product Name
Protein G-UNLB
Product Synonym Names
Protein G - Purified, Unlabeled Antibody; Protein G
Clonality
Polyclonal
Form/Format
Purified (UNLB) Protein G
Specificity
Protein G binds the Fc portion of IgGs from many species including all human, and mouse (IgG1, weakly) subclasses. It does not bind IgM, IgA, IgE or serum albumin.
Preparation and Storage
The purified Protein G is supplied as either 1.0 mg or 5.0 mg in lyophilized form. No BSA, preservatives or amine-containing buffer salts have been added. Store up to one year at -20 degree C; or after reconstitution in phosphate buffered saline to highest concentration possible, store up to one month at 2-8 degree C.
Protein G-HRP conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No other preservatives have been added. Store at 2-8 degree C or long-term at -20 degree C.
Reagents are stable for the period shown on the label if stored as directed.
Source
Recombinant, from Streptococcus Strain G148, produced in E Coli.
Mr/Structure
22 KD. Protein G is a monomeric protein lacking cystine residues; on SDS gel, it migrates to 32KD.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by FPLC and ELISA to conform to the characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-Protein G antibody
Immunoprecipitation
Application Notes for anti-Protein G antibody
Research Application(s): Gamma immunoglobulin capture
Conjugation on solid phase or others to detect gamma immunoglobulin

Product References and Citations for anti-Protein G antibody

1. Saegerman, C., L. De Waele, D. Gilson, J. Godfroid, P. Thiange, P. Michel, B. Limbourg, T.K. Vo, J. Limet, J.J. Letesson, and D. Berkvens. 2004. Vet. Microbiol. 100:91.
2. Qi, Y., Z. Yan, and J. Huang. 2001. J. Biochem. Biophys. Methods 49:263.
3. Sugiura, T., H. Imahawa, and T. Kondo. 2000. J. Chromatogr. B. Biomed. Sci. Appl. 742:327.
4. Yan. Z., and J. Huang. 2000. J. Immunol. Methods 237:203.
5. Aybay, C., and T. Imir. 2000. J. Immunol. Methods 233:77.
6. Bastisa-Corcuera, F.D., K.H. Nielsen, and L.B. Corbeil. 1999. Vet. Immunol. Immunopathol. 71:143.
7. And, Q., G. Luo, J. Ou, and W.S. Yeung. 1999. J. Chromatogr. A. 848:139.
8. Goward CR, Murphy JP, Atkinson T, Barstow DA Expression and purification of a truncated recombinant streptococcal protein G. Biochem J. 1990 Apr 1;267(1):171-7.
9. Guss B, Eliasson M, Olsson A, Uhlén M, Frej AK, Jornvall H, Flock JI, Lindberg M. Structure of the IgG-binding regions of streptococcal protein G. EMBO J. 1986 Jul;5(7):1567-75.

Other Notes

Small volumes of anti-Protein G antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Protein G. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000