NDRG1 Active Protein

Name: NDRG1 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203387
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203387

N-myc downstream regulated gene (NDRG)-1 is one of 4 members of the NDRG alpha/beta-hydrolase family. This protein is classified in databases as a tumor suppressor and heavy metal-response protein. Its functions include cell-cycle regulation, cellular… More details

$210 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NDRG1, Active Protein

Full Product Name

NDRG1, 1-394aa, Human, His tag, E Coli (Bioactivity Validated)

Product Gene Name

NDRG1 active protein

Product Synonym Names

N-myc downstream regulated 1; CAP43; CMT4D; DRG1; GC4; HMSNL; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5; N-myc downstream regulated 1 TDD5; 42 kDa; anti-GC4; cmt4d; Differentiation related gene1 protein; Drg 1; hmsnl; Human mRNA for RTP complete cds; NDRG1; tdds; N myc downstream regulated gene 1; N myc downstream regulated gene 1 protein; Ndr 1; ndr1; NDRG 1; NDRG1 protein; Protein nickel specific induction protein; Nickel specific induction protein Cap43; nmsl; Nmyc downstream regulated; reduced in tumor; reducin; Nmyc downstream regulated gene1; Nmyc downstream regulated gene1 protein; Protein regulated by oxygen 1; Protein regulated by oxygen1; proxy1; Reducing agents and tunicamycin responsive protein; tunicamycin-responsive protein

Sequence

MSREMQDVDL AEVKPLVEKG ETITGLLQEF DVQEQDIETL HGSVHVTLCG TPKGNRPVIL TYHDIGMNHK TCYNPLFNYE DMQEITQHFA VCHVDAPGQQ DGAASFPAGY MYPSMDQLAE MLPGVLQQFG LKSIIGMGTG AGAYILTRFA LNNPEMVEGL VLINVNPCAE GWMDWAASKI SGWTQALPDM VVSHLFGKEE MQSNVEVVHT YRQHIVNDMN PGNLHLFINA YNSRRDLEIE RPMPGTHTVT LQCPALLVVG DSSPAVDAVV ECNSKLDPTK TTLLKMADCG GLPQISQPAK LAEAFKYFVQ GMGYMPSASM TRLMRSRTAS GSSVTSLDGT RSRSHTSEGT RSRSHTSEGT RSRSHTSEGA HLDITPNSGA AGNSAGPKSM EVSCLEHHHH HH

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1 mM PMSF, 10% glycerol

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

43.9 kDa (402aa), confirmed by MALDI-TOF.

OMIM

D87953 mRNA

3D Structure

ModBase 3D Structure for Q92597

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Biological Activity

The ED50 for this effect is 0.5 - 1.5 ng/ml. Measured in a cell proliferation assay using MCF7 cell.

Assay

1. Cell line: MCF7 (Human breast adenocarcinoma cell)
2. Maintenance Condition: RPMI 1640 containing 10% FBS
3. Assay medium: serum free RPMI 1640
4. Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul)
5. Incubation time : 40 hrs (after sample treatment)
6. Concentration range: 0.19ng/ml - 50ng/ml
7. Detection method : Brdu assay

Product Categories/Family for NDRG1 active protein

Cancer

Applications Tested/Suitable for NDRG1 active protein

SDS-PAGE

Product References and Citations for NDRG1 active protein

Richardson DR., et al. (2008) Biochim Biophys Acta. 1783 (10):1981-92.; Costa M., et al. (2008) Carcinogenesis. 29 (1):2-8.;

Other Notes

Small volumes of NDRG1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NDRG1 purchase

MBS203387 is an active protein targeting NDRG1. Also known as NDRG1, 1-394aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NDRG1. It may not necessarily be applicable to this product.

NCBI GI #

37655183

NCBI GeneID

10397

NCBI Accession #

NP_006087

UniProt Primary Accession #

Q92597

UniProt Secondary Accession #

O15207; Q6IBG2; Q9NYR6; Q9UK29; B3KR80; B7Z446

UniProt Related Accession #

Q92597

GenBank Nucleotide #

NM_006096.3

NCBI Official Full Name

protein NDRG1 isoform 1

NCBI Official Synonym Full Names

N-myc downstream regulated 1

NCBI Official Symbol

NDRG1

NCBI Official Synonym Symbols

GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1

UniProt Protein Name

Protein NDRG1

UniProt Synonym Protein Names

Differentiation-related gene 1 protein; DRG-1; N-myc downstream-regulated gene 1 protein; Nickel-specific induction protein Cap43; Reducing agents and tunicamycin-responsive protein; RTP; Rit42

Protein Family

Protein

UniProt Gene Name

NDRG1

UniProt Synonym Gene Names

CAP43; DRG1; RTP; DRG-1; RTP

NCBI Protein Information

protein NDRG1

NCBI Summary for NDRG1

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

UniProt Comments for NDRG1

Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.

Research Articles on NDRG1

1. Compared with the normal term pregnancies, the expression of both NDRG1 mRNA and protein were significantly high in placentas from preeclampsia, and the expression of NDRG1 in early-onset preeclampsia was higher than that in late-onset preeclampsia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NDRG1 active protein

Reference Product	PubMed Publications
MYC active protein	>317 publications with NDRG1 and MYC
SGK1 active protein	>34 publications with NDRG1 and SGK1
HIF1A active protein	>22 publications with NDRG1 and HIF1A
TP53 active protein	>7 publications with NDRG1 and TP53
SH3TC2 active protein	>6 publications with NDRG1 and SH3TC2
MYCN active protein	>5 publications with NDRG1 and MYCN
WISP1 active protein	>4 publications with NDRG1 and WISP1
ARNT active protein	>1 publications with NDRG1 and ARNT

Pathways associated with NDRG1 active protein

Products by Pathway	Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram
TP53 Regulates Transcription Of Cell Death Genes Pathway antibodies	TP53 Regulates Transcription Of Cell Death Genes Pathway Diagram
TP53 Regulates Transcription Of Several Additional Cell Death Genes Whose Specific Roles In P53-dependent Apoptosis Remain Uncertain Pathway antibodies	TP53 Regulates Transcription Of Several Additional Cell Death Genes Whose Specific Roles In P53-dependent Apoptosis Remain Uncertain Pathway Diagram
Transcriptional Regulation By TP53 Pathway antibodies	Transcriptional Regulation By TP53 Pathway Diagram
Validated Targets Of C-MYC Transcriptional Repression Pathway antibodies	Validated Targets Of C-MYC Transcriptional Repression Pathway Diagram

Diseases associated with NDRG1 active protein

Disease Name	Pubmed Publications
Neoplasm Metastasis Antibodies	>55 publications with NDRG1 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>38 publications with NDRG1 and Breast Neoplasms
Prostatic Neoplasms Antibodies	>34 publications with NDRG1 and Prostatic Neoplasms
Liver Diseases Antibodies	>24 publications with NDRG1 and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>24 publications with NDRG1 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>19 publications with NDRG1 and Liver Neoplasms
Neoplasms, Experimental Antibodies	>17 publications with NDRG1 and Neoplasms, Experimental
Lung Neoplasms Antibodies	>14 publications with NDRG1 and Lung Neoplasms
Glioblastoma Antibodies	>10 publications with NDRG1 and Glioblastoma
Cell Transformation, Neoplastic Antibodies	>8 publications with NDRG1 and Cell Transformation, Neoplastic

Organs/Tissues associated with NDRG1 active protein

Organ/Tissue Name	Pubmed Publications
Prostate Antibodies	>51 publications with NDRG1 and Prostate
Nerve Antibodies	>42 publications with NDRG1 and Nerve
Blood Antibodies	>38 publications with NDRG1 and Blood
Brain Antibodies	>37 publications with NDRG1 and Brain
Liver Antibodies	>34 publications with NDRG1 and Liver
Lung Antibodies	>25 publications with NDRG1 and Lung
Vascular Antibodies	>25 publications with NDRG1 and Vascular
Muscle Antibodies	>19 publications with NDRG1 and Muscle
Kidney Antibodies	>18 publications with NDRG1 and Kidney
Lymph Node Antibodies	>17 publications with NDRG1 and Lymph Node