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Streptavidin HRP Conjugate Reagent – ELISA Grade

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS689542

MBS689542 is a reagent targeting Streptavidin HRP Conjugate. Also known as Streptavidin HRP Conjugate (ELISA Grade). More details

$270 / 1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Streptavidin HRP Conjugate, Reagent
Full Product Name
Streptavidin HRP Conjugate (ELISA Grade)
Form/Format
Lyophilized
1.0 mg/ml of lyophilized stretavidin (E1% at 280 nm = 32.0)
(Product has been overfilled to ensure maximum recovery)
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preparation and Storage
Store lyophilized material at 2-8°C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid. Prepare working dilution prior to use and then discard.
Quality Manufacturing Systems
Manufactured under ISO QSR (Quality System Requirements) guidelines, compliant with ISO 13485:2016.
Label
Horseradish Peroxidase (HRP)
Preservative
0.1% (v/v) ProClin 150 - Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase
Reconstitution
Rehydrate with 1.1 ml of deionized water. Allow reconstituted product to stand at least 30 minutes at room temperature prior to dilution. If necessary centrifuge to remove any particulates.
Testing Results
Test: Appearance
Specifications: Reddish powder
Results: PASS

Test: ELISA
Specifications: Dilution giving A450 reading of ≥ 1.0 when tested against Biotinylated antibody coated at 2 ug/ml
Results: 1:315,000

Product Categories/Family for Streptavidin HRP Conjugate reagent

ELISA & Western Blot Reagents
Applications Tested/Suitable for Streptavidin HRP Conjugate reagent
ELISA (EIA); Western Blot (WB); Immunohystochemistry (IHC); CL
Application Notes for Streptavidin HRP Conjugate reagent
This conjugate is designed to be used in the detection of biotinylated primary antibodies.

The optimal working dilution should be determined by the investigator. Suggested starting dilution(s):
• 1:2,000-1:20,000 for Immunohistochemistry
• 1:1,000-1:50,000 for ELISA/Western Blot

Other Notes

Small volumes of Streptavidin HRP Conjugate reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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