Rat Anti-TIM-2 Monoclonal Antibody – Cat# MBS690187

T-Cell Immunoglobulin Mucin-2 (TIM-2) Antibody – Rat Monoclonal

Name: TIM-2 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS690187
Price: 875 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690187

TIM-2 is a type I membrane protein and is a member of the TIM protein family. TIM-2 has an immunoglobulin variable region-like domain followed by a mucin-like domain in its extracellular region.This protein family is characterized by their conserved… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

T-cell Immunoglobulin Mucin-2 (TIM-2), Monoclonal Antibody

Full Product Name

Rat Anti-Mouse T-cell Immunoglobulin Mucin-2 (TIM-2)

Product Gene Name

anti-TIM-2 antibody

Product Synonym Names

Anti-mouse TIM-2 [T-cell Immunoglobulin Mucin-2] (#5B23)

Clonality

Monoclonal

Isotype

IgG2

Clone Number

(5B23)

Host

Rat

Species Reactivity

Mouse

Form/Format

The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at

Purity/Purification

Protein A/G affinity chromatography

Specificity

This antibody detects mouse of TIM-2 in Western blotting. This antibody does not cross-react with mouse TIM-1 and TIM-3.

Preparation and Storage

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Molecular Weight

33,504 Da

Chromosome Location

Chromosome: 11; NC_000077.6 (46668960..46707104, complement). Location: 11 B1.1; 11

3D Structure

ModBase 3D Structure for Q8R183

Antibody Generation

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant TIM-2. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.

Reconstitution

Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-TIM-2 antibody

Western Blot (WB)

Application Notes for anti-TIM-2 antibody

1. Western blotting: Yes (1:500-1000)

Other Notes

Small volumes of anti-TIM-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIM-2 purchase

MBS690187 is an antibody targeting TIM-2. Also known as Rat Anti-Mouse T-cell Immunoglobulin Mucin-2 (TIM-2). Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for TIM-2. It may not necessarily be applicable to this product.

NCBI GI #

238624136

NCBI GeneID

171284

NCBI Accession #

NP_001154827.1

UniProt Primary Accession #

Q8R183

UniProt Secondary Accession #

Q8VBW0

UniProt Related Accession #

Q8R183

GenBank Nucleotide #

NM_001161355.1

NCBI Official Full Name

T-cell immunoglobulin and mucin domain-containing protein 2

NCBI Official Synonym Full Names

T cell immunoglobulin and mucin domain containing 2

NCBI Official Symbol

Timd2

NCBI Official Synonym Symbols

G9D3; Tim2; TIM-2; C78111; AA816106; AU019358; AU019662

UniProt Protein Name

T-cell immunoglobulin and mucin domain-containing protein 2

UniProt Synonym Protein Names

T-cell immunoglobulin mucin receptor 2; TIM-2; T-cell membrane protein 2

UniProt Gene Name

Timd2

UniProt Synonym Gene Names

Tim2; TIMD-2; TIM-2

UniProt Entry Name

TIMD2_MOUSE

NCBI Protein Information

T-cell immunoglobulin and mucin domain-containing protein 2; TIMD-2; T-cell membrane protein 2; T-cell immunoglobulin mucin receptor 2; T-cell immunoglobulin and mucin domain containing 2

UniProt Comments for TIM-2

Function: Probable receptor for SEMA4A involved in the regulation of T-cell function. The interaction with SEMA4A enhances T-cell activation. Ref.3

Subunit structure: Homodimer. Ref.4

Subcellular location: Cell membrane; Single-pass type I membrane protein Ref.4.

Miscellaneous: Belongs to the T-cell and airway phenotype regulator (Tapr) locus, a single chromosomal region that confers reduced T-helper type 2 responsiveness and protects against airway hyperactivity (AHR), the hallmark of human asthma. The human genomic locus appears to lack the Timd2 gene.

Sequence similarities: Belongs to the immunoglobulin superfamily. TIM family.Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Research Articles on TIM-2

1. We concluded that pro-apoptotic effects on Th1 population through galectin-9-Tim-3 pathway and the up-regulation of Tim-2 on Th2 cells might be critical to Th2-biased response of host with schistosomiasis japonica.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-TIM-2 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>7 publications with TIM-2 and Inflammation
Liver Diseases Antibodies	>3 publications with TIM-2 and Liver Diseases
Kidney Diseases Antibodies	>2 publications with TIM-2 and Kidney Diseases
Acute Kidney Injury Antibodies	>1 publications with TIM-2 and Acute Kidney Injury
Liver Failure, Acute Antibodies	>1 publications with TIM-2 and Liver Failure, Acute
Renal Insufficiency Antibodies	>1 publications with TIM-2 and Renal Insufficiency
Brain Diseases Antibodies	>1 publications with TIM-2 and Brain Diseases
Drug-Induced Liver Injury Antibodies	>1 publications with TIM-2 and Drug-Induced Liver Injury

Organs/Tissues associated with anti-TIM-2 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>7 publications with TIM-2 and Liver
Kidney Antibodies	>4 publications with TIM-2 and Kidney
Spleen Antibodies	>2 publications with TIM-2 and Spleen
Lung Antibodies	>2 publications with TIM-2 and Lung