Rat Anti-DKK-3 Monoclonal Antibody – Cat# MBS690525

DKK-3 Antibody – Rat Monoclonal

Name: DKK-3 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS690525
Price: 875 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690525

Dkk3 is a member of the Dickkopf family of secreted cysteine-rich domain-containing proteins. Some members of this family are known to regulate developmental processes by modulating the signaling of Wnt family members. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Dkk-3, Monoclonal Antibody

Full Product Name

Rat Anti-Mouse Dkk-3

Product Gene Name

anti-Dkk-3 antibody

Product Synonym Names

Dkk3; C87148; AW061014

Clonality

Monoclonal

Isotype

IgG2

Clone Number

9E12

Host

Rat

Species Reactivity

Mouse

Form/Format

Lyophilized

Preparation and Storage

Lyophilized samples are stable for 1 year from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Molecular Weight

38,388 Da

Chromosome Location

Chromosome: 7; NC_000073.6 (112116019..112159121, complement). Location: 7 F1; 7

3D Structure

ModBase 3D Structure for Q9QUN9

Preparation

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of Dickkopf homolog-3 (Dkk-3).

Reconstitution

Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-Dkk-3 antibody

Western Blot (WB)

Application Notes for anti-Dkk-3 antibody

WB: 1:500-1:2000
Optimal dilution should be determined by each laboratory for each application.
Avoid repeated freeze and thaw cycles.

Other Notes

Small volumes of anti-Dkk-3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Dkk-3 purchase

MBS690525 is an antibody targeting Dkk-3. Also known as Rat Anti-Mouse Dkk-3. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for Dkk-3. It may not necessarily be applicable to this product.

NCBI GI #

7657027

NCBI GeneID

50781

NCBI Accession #

NP_056629.1

UniProt Primary Accession #

Q9QUN9

UniProt Related Accession #

Q9QUN9

GenBank Nucleotide #

NM_015814.2

NCBI Official Full Name

dickkopf-related protein 3

NCBI Official Synonym Full Names

dickkopf homolog 3 (Xenopus laevis)

NCBI Official Symbol

Dkk3

NCBI Official Synonym Symbols

C87148; AW061014

UniProt Protein Name

Dickkopf-related protein 3

Protein Family

Dickkopf-related protein

UniProt Gene Name

Dkk3

UniProt Synonym Gene Names

Dickkopf-3; Dkk-3; mDkk-3

UniProt Entry Name

DKK3_MOUSE

NCBI Protein Information

dickkopf-related protein 3; dkk-3; mDkk-3; dickkopf-3

UniProt Comments for Dkk-3

Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease

By similarity.

Subunit structure: Interacts with LRP5 and LRP6

By similarity.

Subcellular location: Secreted.

Tissue specificity: Highest expression in brain, eye and heart.

Domain: The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6

By similarity.

Sequence similarities: Belongs to the dickkopf family.

Research Articles on Dkk-3

1. This is the first report of role of DKK3 in the testis and DKK3 mediated regulation of spermatogenesis via WNT-4/beta-CATENIN modulation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Dkk-3 antibody

Reference Product	PubMed Publications
Sfrp2 antibody	>30 publications with Dkk-3 and Sfrp2
Wif1 antibody	>27 publications with Dkk-3 and Wif1
Sfrp5 antibody	>24 publications with Dkk-3 and Sfrp5
Sfrp4 antibody	>23 publications with Dkk-3 and Sfrp4
Lrp6 antibody	>7 publications with Dkk-3 and Lrp6
Frzb antibody	>6 publications with Dkk-3 and Frzb
Kremen1 antibody	>5 publications with Dkk-3 and Kremen1
Lrp5 antibody	>4 publications with Dkk-3 and Lrp5
Kremen2 antibody	>2 publications with Dkk-3 and Kremen2

Diseases associated with anti-Dkk-3 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>116 publications with Dkk-3 and Neoplasms
Carcinoma Antibodies	>51 publications with Dkk-3 and Carcinoma
Adenocarcinoma Antibodies	>25 publications with Dkk-3 and Adenocarcinoma
Prostatic Neoplasms Antibodies	>19 publications with Dkk-3 and Prostatic Neoplasms
Lung Neoplasms Antibodies	>16 publications with Dkk-3 and Lung Neoplasms
Colorectal Neoplasms Antibodies	>12 publications with Dkk-3 and Colorectal Neoplasms
Skin Diseases Antibodies	>10 publications with Dkk-3 and Skin Diseases
Breast Neoplasms Antibodies	>10 publications with Dkk-3 and Breast Neoplasms
Carcinoma, Hepatocellular Antibodies	>9 publications with Dkk-3 and Carcinoma, Hepatocellular
Kidney Diseases Antibodies	>7 publications with Dkk-3 and Kidney Diseases

Organs/Tissues associated with anti-Dkk-3 antibody

Organ/Tissue Name	Pubmed Publications
Prostate Antibodies	>24 publications with Dkk-3 and Prostate
Eye Antibodies	>22 publications with Dkk-3 and Eye
Lung Antibodies	>20 publications with Dkk-3 and Lung
Embryonic Tissue Antibodies	>14 publications with Dkk-3 and Embryonic Tissue
Brain Antibodies	>13 publications with Dkk-3 and Brain
Heart Antibodies	>10 publications with Dkk-3 and Heart
Liver Antibodies	>9 publications with Dkk-3 and Liver
Skin Antibodies	>8 publications with Dkk-3 and Skin
Bladder Antibodies	>7 publications with Dkk-3 and Bladder
Connective Tissue Antibodies	>5 publications with Dkk-3 and Connective Tissue