Angiopoietin-Like Protein 3 (ANGPTL3) Antibody – Rat Monoclonal

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins.Mature mouse ANGPTL3 contains an N-terminal coiled coil domain and a C-terminal fibrinogen like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Full length ANGPTL3 circulates in the plasma as do the proteolytically separated Nand C-terminal fragments containing the coiled coil domain and fibrinogenlike domains, respectively. ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species and can form weakly associated noncovalent multimers in vitro. ANGPTL3 directly inhibits lipoprotein lipase (LPL), an enzyme responsible for hydrolyzing circulating triglycerides. This activity requires a putative heparinbinding motif that is N-terminal to the coiled coil domain. Proteolytic removal of the fibrinogenlike domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo. ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake. ANGPTL3 knockoutmice are hypolipidemic and have elevated LPL activity. ANGPTL3 expression in vivo is upregulated by LXR agonists and downregulated by insulin, leptin, and TRbeta agonists. Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice, ANGPTL3 does not bind Tie1 or Tie2 but its fibrinogenlike domain interacts with integrin alphaVbeta3 to induce endothelial cell adhesion, migration, and neovascularization. ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells. Mature mouse ANGPTL3 shares 22%-30% amino acid (aa) sequence identity with ANGPTL 1, 2, 4, 6, and 7. It shares 77% aa sequence identity with human ANGPTL3.

Small volumes of anti-Angptl3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS690571 is an antibody targeting Angptl3. Also known as Rat Anti-Mouse Angiopoietin-like protein 3. Reactive in Mouse. Host: Rat.