LEC26 Antibody – Rat Monoclonal

Monoclonal

IgG2b

IgG2b, LA102

Rat

Mouse

Lyophilized

Protein G purified

LA102 reacts with mouse lymphatic vessels except the thoracic duct and the marginal sinus of lymph nodes, but not with any blood vessels. LA102 recognized a protein of around 26 kDa. The antigen recognized by LA102 was localized on both luminal and abluminal lymphatic endothelial cell membranes. Besides lymphatics, some lymphoid cells are also recognized.

The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C.

With collagenase- and neuraminidase-treated mouse benign lymphangiomas

The antibody was produced by a rapid differential immunization of DA rats with collagenase- and neuraminidase-treated mouse benign lymphangiomas (licensed from Tokyo Women's Medical University, Japan). Two novel rat monoclonal antibodies (LA102 and LA5) were generated to identify mouse lymphatic vessels and blood vessels, respectively. Both antibodies were characterized as to the morphological and functional specificities of endothelial cells of both types of vessels. The antibodies were produced by a rapid differential immunization of DA rats with collagenase- and neuraminidase-treated mouse lymphangioma tissues. LA102 specifically reacted with mouse lymphatic vessels except the thoracic duct and the marginal sinus of lymph nodes, but not with any blood vessels. LA102 recognized a protein of 25-27 kDa. Neither antibody recognized any currently identified lymphatic or vascular endothelial cell antigens. Immunoelectron microscopy revealed that the antigens recognized by LA102 were localized on both luminal and abluminal endothelial cell membranes of each vessel type. Interestingly, LA102 immunoreactivity was strongly expressed on pinocytic or transport vesicle membrane in the cytoplasm of lymphatic endothelium. Besides endothelial cells, both antibodies also recognized some types of lymphoid cells. Since, the LA102 antigen molecule is expressed on some lymphoid cells, it may play important roles in the migration of lymphoid cells and in some transport mechanisms through lymphatic endothelial cells.

PBS

Manufactured in an ISO 9001:2008 Certified Laboratory.