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GM-CSF Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS691548

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), a 14.6 kDa protein consisting of 127 amino acid residues (Ala18-Glu144) and fused to a C-terminal His-tag (6x His), is a potent species specific stimulator of bone marrow cells and several other cell… More details

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Product Overview

Online Datasheet in PDF
Product Name
GM-CSF, Active Protein
Full Product Name
Human GM-CSF
Product Gene Name
GM-CSF active protein
Product Synonym Names
Recombinant Human Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF); Colony-stimulating factor; GM-CSF
Sequence
N-Terminal Sequence: MAPARSPST
Host
Insect Cells
Form/Format
Lyophilized; PBS
Purity/Purification
> 98% by SDS-PAGE & silver stain
Preparation and Storage
The lyophilized powder although stable at room temperature for 3 weeks, is best stored desiccated at -20°C.
Reconstituted GM-CSF should be stored in working aliquots at –20°C. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).
Molecular Weight
~15.0 - 18.0 kDa
Sequence Positions
APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEP TCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFE SFKENLKDFLLVIPFDCWEPVQETRHHHHHH
Chromosome Location
Chromosome: 5; NC_000005.10 (132073792..132076170). Location: 5q31.1
OMIM
138960
3D Structure
ModBase 3D Structure for P04141
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
N Terminal Sequence
MAPARSPST
Length (aa)
135
Reconstitution
The lyophilized rh GM-CSF is soluble in water and most aqueous buffers and can be reconstituted in water to a concentration of 0.1 mg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.
Biological Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells [Kitamura T et al, J Cell Physiol, 1989]. The ED50 for this effect is typically <0.1ng/ml corresponding to a specific activity of >1 x 107units/mg.

Product Categories/Family for GM-CSF active protein

Cytokines & Growth Factors
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.

Testing Data of GM-CSF active protein

Human GM-CSF Active Protein – Cat# MBS691548

Testing Data #2 of GM-CSF active protein

Human GM-CSF Active Protein, Testing Data #2 – Cat# MBS691548

Product References and Citations for GM-CSF active protein

1. Martinez-Moczygemba and Huston (2003) J. Allergy Clin Immunol 112:653 2. Barreda et al, (2004) Dev Comp Immunol 28:509 3. Eksioglu et al, (2007) Exp Hematol 35:1163 4. Cao Y, (2007) J Clin Invest 117:2362 5. Fleetwood et al, (2005) Crit Rev Immunol 25:405 6. Diederichs et al, (1991) Science 254:1779 7. Gough et al, (1984) Nature 309:763

Other Notes

Small volumes of GM-CSF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GM-CSF purchase

MBS691548 is an active protein targeting GM-CSF. Also known as Human GM-CSF. Host: Insect Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for GM-CSF. It may not necessarily be applicable to this product.

NCBI GI #
27437030
NCBI GeneID
1437
NCBI Accession #
NP_0000749
UniProt Primary Accession #
P04141
UniProt Secondary Accession #
Q14CE8; Q2VPI8; Q8NFI6
UniProt Related Accession #
P04141
GenBank Nucleotide #
NM_000758
NCBI Official Full Name
granulocyte-macrophage colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 2 (granulocyte-macrophage)
NCBI Official Symbol
CSF2
NCBI Official Synonym Symbols
GMCSF
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Synonym Protein Names
Colony-stimulating factor; CSF; Molgramostin; Sargramostim
Protein Family
Granulocyte colony-stimulating factor receptor
UniProt Gene Name
CSF2
UniProt Synonym Gene Names
GMCSF; GM-CSF; CSF
UniProt Entry Name
CSF2_HUMAN
NCBI Protein Information
granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim

NCBI Summary for GM-CSF

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
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UniProt Comments for GM-CSF

Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Subunit structure: Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits. Ref.13

Subcellular location: Secreted.

Polymorphism: Variant Ile-117 may be a risk factor for atopic asthma.

Pharmaceutical use: Available under the names Leukine (Immunex) and Leucomax (Novartis). Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Sequence similarities: Belongs to the GM-CSF family.
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Research Articles on GM-CSF

1. The data presented suggest that -677C/A in the promoter region of the GM-CSF gene does not play a role in acne pathogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GM-CSF active protein

 Reference Product  PubMed Publications
 IL4 active protein  >17 publications with GM-CSF and IL4
 IL3 active protein  >13 publications with GM-CSF and IL3
 IL5 active protein  >10 publications with GM-CSF and IL5
 CSF2RB active protein  >8 publications with GM-CSF and CSF2RB
 CSF2RA active protein  >5 publications with GM-CSF and CSF2RA
 STAT3 active protein  >2 publications with GM-CSF and STAT3
 FOS active protein  >2 publications with GM-CSF and FOS
 JAK2 active protein  >1 publications with GM-CSF and JAK2
 SDC2 active protein  >1 publications with GM-CSF and SDC2

Pathways associated with GM-CSF active protein

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram

Diseases associated with GM-CSF active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >32 publications with GM-CSF and Inflammation
 Necrosis Antibodies  >23 publications with GM-CSF and Necrosis
 Neoplasms Antibodies  >21 publications with GM-CSF and Neoplasms
 Lung Diseases Antibodies  >8 publications with GM-CSF and Lung Diseases
 Breast Neoplasms Antibodies  >5 publications with GM-CSF and Breast Neoplasms
 Carcinoma Antibodies  >3 publications with GM-CSF and Carcinoma
 Leukemia, Myeloid, Acute Antibodies  >3 publications with GM-CSF and Leukemia, Myeloid, Acute
 Lung Neoplasms Antibodies  >3 publications with GM-CSF and Lung Neoplasms
 Arthritis, Rheumatoid Antibodies  >2 publications with GM-CSF and Arthritis, Rheumatoid
 Neoplasm Invasiveness Antibodies  >2 publications with GM-CSF and Neoplasm Invasiveness

Organs/Tissues associated with GM-CSF active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >36 publications with GM-CSF and Blood
 Bone Antibodies  >13 publications with GM-CSF and Bone
 Lung Antibodies  >9 publications with GM-CSF and Lung
 Intestine Antibodies  >2 publications with GM-CSF and Intestine
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